Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

329 Products
Data Quality
  • 18
Type
  • 329
    Enzyme
Gene
  • 4
  • 2
  • 2
  • 2
  • 1
Species
  • 8
  • 4
  • 3
  • 2
  • 1
Application
  • 119
  • 66
  • 33
  • 32
  • 24
Host
  • 10
  • 4
Protein Type
  • 9
Format
  • 18
  • 4

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
qPCR Instruments:
- ABI® 7500 Viia™
- QuantStudio
- BioGene InSyte™
- Illumina Eco
- Stratagene® Mx3000, Mx3005, Mx4000
- Analytikjena qTower Series
Catalog No. ABIN4219207
$189.26
4 x 1.25 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
qPCR Instruments: Any qPCR instrument that supports multiplex reactions
Catalog No. ABIN4219208
$189.26
4 x 1.25 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Quantitative real-time PCR
Components:
Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Storage:
-20 °C
Specificity:
qPCR Instruments:
- ABI® 7000, 7300, 7700, 7900, 7900HT,
- StepOnePlus™
- StepOne™
- OpenArray
- PRISM™ Sequencing Detection Series
- Biometra TOptical
- Fluidigm BioMarkTM
- Wafergene SmartChip System
- TianLong TL998 System
Publications
  • Kadam, Chuan: "Erratum to: Rectocutaneous fistula with transmigration of the suture: a rare delayed complication of vault fixation with the sacrospinous ligament." in: International urogynecology journal, Vol. 27, Issue 3, pp. 505, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Park, Ju, Park, Song, Shin, Lee, Jeong, Yu, Lee, Cho, Kim, Kim, Kim, Park, Chun: "PPARγ neddylation essential for adipogenesis is a potential target for treating obesity." in: Cell death and differentiation, Vol. 23, Issue 8, pp. 1296-311, 2016 (Pubmed)
  • Wood, Cox, Phelps, Lai, Poddar, Talbot, Mu: "Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome." in: Scientific reports, Vol. 6, Issue , pp. 19857, 2016 (Pubmed)
  • Ren, Sun, Kang, Zhao, Feng, Ren, Han, Yang: "Responsiveness of soil nitrogen fractions and bacterial communities to afforestation in the Loess Hilly Region (LHR) of China." in: Scientific reports, Vol. 6, Issue , pp. 28469, 2016 (Pubmed)
  • Zhao, Zhang, Guo, Li, Han, Song, Wang, Li, Li: "Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression in Dioscorea opposita." in: BioMed research international, Vol. 2016, Issue , pp. 3089584, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219210
$189.26
4 x 1.25 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
qPCR Instruments:
- BioRad® CFX96
- CFX384
- Chromo4™
- CFX Connect™
- Opticon 2
- MiniOpticon™
- Roche LightCycler® (2.0, 1.5, 480, 1536, Nano)
- MJ Research Opticon™
- Opticon™ 2
- Chromo® 4
- Eppendorf® Realplex 4
- BioGene SynChron™
- Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
- Eppendorf Mastercycler® realplex (s, 4 , 4s)
- Pro (S, 384)
- Nexus (gradient, eco, flat)
- Cepheid SmartCycler®
- GeneXpert
- Enigma® ML
- Idaho LightScanner® (24, 32)
- RapidCycler®2
- R.A.P.I.D (LT, LT Food)
- RAZOR EX
- JBAIDS
- Qiagen Rotor-Gene™ (Q, 6000)
- Takara Dice™
-T hermo Scientific PikoReal
- DNA-Technology DT96
- DTlite
- DT-322
- Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
- Bioneer ExicyclerTM
Catalog No. ABIN4219209
$189.26
4 x 1.25 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Quantitative real-time PCR
Components:
Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Storage:
-20 °C
Specificity:
qPCR Instruments:
- BioRad® CFX96
- CFX384
- Chromo4™
- CFX Connect™
- Opticon 2
- MiniOpticon™
- Roche LightCycler® (2.0, 1.5, 480, 1536, Nano)
- MJ Research Opticon™
- Opticon™ 2
- Chromo® 4
- Eppendorf® Realplex 4
- BioGene SynChron™
- Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
- Eppendorf Mastercycler® realplex (s, 4 , 4s)
- Pro (S, 384)
- Nexus (gradient, eco, flat)
- Cepheid SmartCycler®
- GeneXpert
- Enigma® ML
- Idaho LightScanner® (24, 32)
- RapidCycler®2
- R.A.P.I.D (LT, LT Food)
- RAZOR EX
- JBAIDS
- Qiagen Rotor-Gene™ (Q, 6000)
- Takara Dice™
-T hermo Scientific PikoReal
- DNA-Technology DT96
- DTlite
- DT-322
- Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
- Bioneer ExicyclerTM
Publications
  • Lee, Jeong, Kwon, Van Kien, Lee, Hong, Lee: "Drastic anthocyanin increase in response to PAP1 overexpression in fls1 knockout mutant confers enhanced osmotic stress tolerance in Arabidopsis thaliana." in: Plant cell reports, Vol. 35, Issue 11, pp. 2369-2379, 2016 (Pubmed)
  • Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
  • Kim, Kim, Jung, Lee, Lee, Lee, Lee, You, Chung, Ye: "Potential role of 8-oxoguanine DNA glycosylase 1 as a STAT1 coactivator in endotoxin-induced inflammatory response." in: Free radical biology & medicine, Vol. 93, Issue , pp. 12-22, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Bernaudo, Salem, Qi, Zhou, Zhang, Yang, Rosman, Deng, Ye, Yang, Vanderhyden, Wu, Peng: "Cyclin G2 inhibits epithelial-to-mesenchymal transition by disrupting Wnt/β-catenin signaling." in: Oncogene, Vol. 35, Issue 36, pp. 4816-27, 2016 (Pubmed)
  • Lv, Liu, Zhao, Zhou, Wang, Guo, Fang: "Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein." in: Scientific reports, Vol. 6, Issue , pp. 35751, 2016 (Pubmed)
  • Xu, Song, Li, Tu, Liu, Liu, Wang, Wang, Cui, Liu, Shang, de Rooij, Gao, Li: "H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation." in: Nucleic acids research, Vol. 44, Issue 20, pp. 9681-9697, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Smither, Pang, Brubaker: "Glucagon-Like Peptide-2 Requires a Full Complement of Bmi-1 for Its Proliferative Effects in the Murine Small Intestine." in: Endocrinology, Vol. 157, Issue 7, pp. 2660-70, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219211
$189.26
4 x 1.25 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Quantitative real-time PCR
Concentration:
1000 units/mL
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double stranded, uracil containing DNA.
Catalog No. ABIN3188314
$192.50
300 U
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 6 to 7 Business Days

Application:
Polymerase Chain Reaction
Components:
FlashPfu DNA Polymerase 1 U/μl, 2x flashPfu PCR Buffer, 25 mM MgSO4
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666438
$194.00
250 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, gDNA Remover, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 12 kb.
Catalog No. ABIN5519519
$200.00
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 15 to 19 Business Days

Catalog No. ABIN5684223
$200.00
5 g
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 2 to 3 Business Days

Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
AflII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188181
$202.21
10000 U
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
NcoI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188201
$202.21
10000 U
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Polymerase Chain Reaction
Storage:
-20 °C
Specificity:
With Safe-Green™ 2X PCR Bestaq™ MasterMix, you can
- Reduce PCR set up time
- Eliminate ethidium bromide usage and associated waste disposal costs
- Increase PCR efficiency and reproducibility

The use of Bestaq™ DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Catalog No. ABIN4219179
$202.21
5 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952628
$204.65
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Publications
  • Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
  • Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
  • Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
  • Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
  • Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219182
$208.68
200 x 10 μL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
5xTransScript® All-in-One SuperMix for PCR, 5xTransScript® All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Catalog No. ABIN5519498
$215.00
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 15 to 19 Business Days

Catalog No. ABIN5684239
$215.00
100 μL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 2 to 3 Business Days

Application:
DNA Amplification
Concentration:
10 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
Catalog No. ABIN3188235
$215.15
1000 U
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952629
$217.44
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days

Application:
DNA Modification
Concentration:
>2000 Kunitz U/mg
Catalog No. ABIN2952934
$217.44
15000 U
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days

Application:
DNA Modification
Catalog No. ABIN2952656
$217.44
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days