DNA Amplification

Polymerase enzymes are responsible for replication and formation of new nucleic acid strands. Polymerase enzymes exist in many forms, and fulfill different roles in molecular biology based on template and end product:

  • DNA-dependent DNA polymerases (often referred to as DNA replication enzymes or simply DNA polymerases) recognize a template of single stranded DNA, and anneal one deoxynucleotide at a time to form a complementary strand of DNA. DNA-dependent DNA polymerases are most frequently used for DNA replication in experiments like polymerase chain reaction (PCR).
  • RNA-dependent DNA polymerases (often referred to as reverse transcriptases) recognizes a template of single stranded RNA, and anneal a single deoxynucleotide at a time to form a complete complementary strand of DNA. RNA-dependent DNA-polymerases are frequently used to generate cDNA copies of mRNA transcripts (e.g. when generating a cDNA library via RT-PCR).

More information about our Taq, Pfu and special polymerases.

Type
  • 6
    Enzyme
Application
  • 6
    DNA Amplification (DNA Amp)
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6 Products

Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188193
$59.85
500 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188231
$85.74
500 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
DNA Amplification
Concentration:
1 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
Catalog No. ABIN3188236
$85.74
100 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188210
$150.44
500 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
DNA Amplification
Concentration:
10 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
Catalog No. ABIN3188235
$215.15
1000 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Klenow Fragment (3'→5' Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188198
$85.74
500 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days
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