DNA Polymerase I Large (Klenow) Fragment
DNA Amp
5 U/μL
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- Application
- DNA Amplification (DNA Amp)
- Characteristics
- DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coliDNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5'→3' exonuclease activity. With its inherent 3'→5' exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.
- Components
- Enzyme supplied with 10X Reaction Buffer
- Unit Definition
- One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
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- Comment
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- DNA blunting by fillingin 5'overhangs with unlabeled or labeled dNTPs
- cDNA secondstrand synthesis
- Generate singlestranded DNA probes using random primers
- Sitedirected DNA mutagenesis using synthetic oligonucleotides
- Dideoxy DNA sequencing of single or doublestranded DNA templates
- 3'→5' exonuclease activity can blunt a 3'overhang
- Restrictions
- For Research Use only
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- Concentration
- 5 U/μL
- Buffer
- 25 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50 % (v/v) Glycerol.
- Storage
- -20 °C
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