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Uracil DNA Glycosylase Enzyme

5 U/μL
Catalog No. ABIN3188217
$59.85
Plus shipping costs $45.00 and $24.00 dry ice
1000 U
local_shipping Shipping to: United States
Delivery in 11 to 21 Business Days
  • Characteristics
    E. coli Uracil DNA Glycosylase (UDG) catalyses the release of free uracil via hydrolysis of the N-glycosylic bond between the uracil and sugar from uracil containing single- or double- stranded DNA. This enzyme does not show activity on oligomers of 6 or less bases or on RNA.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of Uracil DNA Glycosylase that catalyzes the release of 60 pmol of uracil per one minute from uracil-containing, double-stranded DNA at 37°C.
  • Comment

    • Prevents carryover contamination in PCR
    • Glycosylase mediated polymorphism detection (GMPD)
    • Sitedirected mutagenesis

    Restrictions
    For Research Use only
  • Concentration
    5 U/μL
    Buffer
    30 mM Tris-HCl ( pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.15 % Tween-20 and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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