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Exonuclease I, E. coli Enzyme

DNA Mod 20 U/μL
Catalog No. ABIN3188195
$46.91
Plus shipping costs $45.00 and $24.00 dry ice
2000 U
local_shipping Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    DNA Modification (DNA Mod)
    Characteristics
    Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3'→5' direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3'-terminus is phosphorylated.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
  • Comment

    • Removal of singlestranded primer oligonucleotides from:
    • PCR mixtures for applications involving sequencing or labelling
    • from nucleic acid mixtures
    • Assaying for the presence of singlestranded DNA with a 3'hydroxyl terminus

    Restrictions
    For Research Use only
  • Concentration
    20 U/μL
    Buffer
    20 mM HEPES ( pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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