Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3'→5' direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3'-terminus is phosphorylated.
Components
Enzyme supplied with 10X Reaction Buffer
Unit Definition
One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.