+1 877 302 8632
+1 888 205 9894 (Toll-free)
[email protected]

Exonuclease I, E. coli

DNA Mod 20 U/μL
Catalog No. ABIN3188195
Plus shipping costs $50.00, dry ice fee $20.00 and VAT
2000 U
Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    DNA Modification (DNA Mod)
    Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3'→5' direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3'-terminus is phosphorylated.
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
  • Comment

    • Removal of singlestranded primer oligonucleotides from:
    • PCR mixtures for applications involving sequencing or labelling
    • from nucleic acid mixtures
    • Assaying for the presence of singlestranded DNA with a 3'hydroxyl terminus

    For Research Use only
  • Concentration
    20 U/μL
    20 mM HEPES ( pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50 % (v/v) Glycerol.
    -20 °C
You are here: