Exonuclease I, E. coli
DNA Mod
20 U/μL
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- Application
- DNA Modification (DNA Mod)
- Characteristics
- Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3'→5' direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3'-terminus is phosphorylated.
- Components
- Enzyme supplied with 10X Reaction Buffer
- Unit Definition
- One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
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- Comment
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- Removal of singlestranded primer oligonucleotides from:
- PCR mixtures for applications involving sequencing or labelling
- from nucleic acid mixtures
- Assaying for the presence of singlestranded DNA with a 3'hydroxyl terminus
- Restrictions
- For Research Use only
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- Concentration
- 20 U/μL
- Buffer
- 20 mM HEPES ( pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50 % (v/v) Glycerol.
- Storage
- -20 °C
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