E. coli DNA Polymerase I

Details for Product No. ABIN3188193, Supplier: Log in to see
Application
DNA Amplification (DNA Amp)
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Characteristics E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3'→5' and 5'→3' exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Comment

  • Generation of labeled DNA probes by nick translation
  • Secondstrand synthesis of cDNA
Restrictions For Research Use only
Concentration 5 U/μL
Buffer 25 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50 % (v/v) Glycerol.
Storage -20 °C