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E. coli DNA Polymerase I Enzyme

DNA Amp 5 U/μL
Catalog No. ABIN3188193
$59.85
Plus shipping costs $45.00 and $24.00 dry ice
500 U
local_shipping Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    DNA Amplification (DNA Amp)
    Characteristics
    E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3'→5' and 5'→3' exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
  • Comment

    • Generation of labeled DNA probes by nick translation
    • Secondstrand synthesis of cDNA

    Restrictions
    For Research Use only
  • Concentration
    5 U/μL
    Buffer
    25 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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