E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3'→5' and 5'→3' exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity.
Components
Enzyme supplied with 10X Reaction Buffer
Unit Definition
One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.