cDNA Synthesis

Complementary DNA (cDNA) is synthesized in a reverse transcription reaction by the enzyme reverse transcriptase from a single-stranded mRNA template. cDNA molecules are commonly used to clone and express eukaryotic genes in prokaryotes since the original genes cannot be readily cloned due to the presence of introns. Our reverse transcriptase and cDNA synthesis premixes provide you with the necessary tools for cDNA first-strand synthesis and PCR amplification specifically from mRNA or total RNA extracts.
Type
  • 21
    Enzyme
Application
  • 21
    cDNA Synthesis (cDNA)
  • 61
  • 32
  • 32
  • 23
21 Products

Application:
cDNA Synthesis
Components:
EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519431
$101.58
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519523
$135.96
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
EasyScript® RT/RI Enzyme Mix, 2xES Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the, product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519428
$141.69
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519500
$178.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219166
$182.79
100 x 20 μL
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, gDNA Remover, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 12 kb.
Catalog No. ABIN5519519
$200.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
5xTransScript® All-in-One SuperMix for PCR, 5xTransScript® All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Catalog No. ABIN5519498
$215.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® All-in-One SuperMix for PCR, RNase-free Water
Storage:
-20 °C
Specificity:
One-tube format for simple and fast setup and reduce pipetting variability.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 12 kb.
Catalog No. ABIN5519497
$231.00
50 reactions
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
EasyScript® One-Step Enzyme Mix, 2x One-Step Reaction Mix, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 4 kb.
Catalog No. ABIN5519430
$256.00
200 reactions
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
5xTransScript® All-in-One SuperMix for PCR, 2xTransTaq® HiFi PCR SuperMix II, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 12 kb.
Catalog No. ABIN5519525
$261.00
1 kit
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® II Reverse Transcriptase, 5xTS II RT Buffer, Anchored Oligo (dT)20 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Increased thermostability for more full-length cDNA products.
Reaction temperature at 42 °C-55 °C.
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb.
Catalog No. ABIN5519516
$265.00
10000 units
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® One-Step Enzyme Mix, 2x One-Step Reaction Mix, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 8 kb.
Catalog No. ABIN5519518
$311.00
200 reactions
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
5x TransScript® All-in-One SuperMix for PCR, RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb.
Catalog No. ABIN5519508
$314.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
C to C Lysis Buffer, Enzyme Mix, gDNA Remover, Reaction Mix, Oligo (dT)/RP Mix, RNase-free Water
Storage:
-20 °C
Catalog No. ABIN5519494
$351.00
25 reactions
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® II RT/RI Enzyme Mix, gDNA Remover, 2xTS II Reaction Mix, Random Primer (0.1 μg/μL), Anchored Oligo(dT)20 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 15 kb.
Catalog No. ABIN5519514
$351.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
5xTransScript® II All-in-One SuperMix for PCR, 5xTransScript® II All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of Oligo(dT)20 Primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Catalog No. ABIN5519507
$365.00
50 reactions
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
5xTransScript® II All-in-One SuperMix for PCR, RNase-free Water
Storage:
-20 °C
Specificity:
One-tube format for simple and fast setup and reduced pipetting variability.
The optimal ratio of oligo(dT)20 primer to random primer(N9) for PCR ready cDNA.
PCR ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 15 kb.
Catalog No. ABIN5519506
$381.00
50 reactions
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
Enzyme Mix, Reaction Mix, Universal miRNA qPCR Primer (10μM), RNase-free Water
Storage:
-20 °C
Specificity:
Optimized enzyme and buffer system for high efficient cDNA synthesis.
One-step Poly(A) tailing and cDNA synthesis.
Catalog No. ABIN5519526
$381.00
20 reactions
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript®-Uni RT/RI Enzyme Mix, gDNA Remover, 2xTS-Uni Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)20 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Broad range reaction temperature (42 °C-65 °C) .
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 20 kb.
Catalog No. ABIN5519495
$395.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
5xTransScript® II All-in-One SuperMix for PCR, 2xTransTaq® HiFi PCR SuperMix II, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 15 kb.
Catalog No. ABIN5519517
$411.00
1 kit
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days
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