cDNA Synthesis

Complementary DNA (cDNA) is synthesized in a reverse transcription reaction by the enzyme reverse transcriptase from a single-stranded mRNA template. cDNA molecules are commonly used to clone and express eukaryotic genes in prokaryotes since the original genes cannot be readily cloned due to the presence of introns. Our reverse transcriptase and cDNA synthesis premixes provide you with the necessary tools for cDNA first-strand synthesis and PCR amplification specifically from mRNA or total RNA extracts.
38 Products
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    Enzyme
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    cDNA Synthesis (cDNA)
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Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219164
$53.38
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Publications
  • Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
  • Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
  • Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
  • Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
  • Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219180
$59.85
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219163
$72.79
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Publications
  • Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
  • Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
Catalog No. ABIN4219169
$92.21
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666450
$94.00
5000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days

Application:
cDNA Synthesis
Components:
EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519431
$100.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains EasyScript™ Plus Reverse Transcriptase.
Publications
  • Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
Catalog No. ABIN4219171
$105.15
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219165
$118.09
100 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519523
$137.46
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Publications
  • Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
  • Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
  • Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
  • Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
  • Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219181
$137.50
100 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
EasyScript® RT/RI Enzyme Mix, 2xES Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the, product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519428
$139.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519500
$174.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Publications
  • Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
Catalog No. ABIN4219170
$176.32
100 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219166
$182.79
100 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, gDNA Remover, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 12 kb.
Catalog No. ABIN5519519
$196.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Publications
  • Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
  • Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
  • Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
  • Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
  • Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219182
$208.68
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
5xTransScript® All-in-One SuperMix for PCR, 5xTransScript® All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Catalog No. ABIN5519498
$210.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains EasyScript™ Plus Reverse Transcriptase.
Publications
  • Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
Catalog No. ABIN4219172
$221.62
100 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
TransScript® All-in-One SuperMix for PCR, RNase-free Water
Storage:
-20 °C
Specificity:
One-tube format for simple and fast setup and reduce pipetting variability.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 12 kb.
Catalog No. ABIN5519497
$225.00
50 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666451
$245.00
20000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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