cDNA Synthesis

Complementary DNA (cDNA) is synthesized in a reverse transcription reaction by the enzyme reverse transcriptase from a single-stranded mRNA template. cDNA molecules are commonly used to clone and express eukaryotic genes in prokaryotes since the original genes cannot be readily cloned due to the presence of introns. Our reverse transcriptase and cDNA synthesis premixes provide you with the necessary tools for cDNA first-strand synthesis and PCR amplification specifically from mRNA or total RNA extracts.
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    cDNA Synthesis (cDNA)
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Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219164
$53.38
25 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.

Application:
cDNA Synthesis
Components:
EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519431
$55.00
10000 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

From RNA template to cDNA in just 15 minutes completely hassle-free.

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Catalog No. ABIN4219180
$59.85
25 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

M-MLV Reverse Transcriptase (RNaseH-) is a recombinant reverse transcriptase expressed and purified from E. Coil. This enzyme can be used in cDNA synthesis with long messenger RNA templates due to the lacking of RNase H activity by point mutation.

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666450
$69.00
5000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Supplier: Log in to see

Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219163
$72.79
25 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.

Application:
cDNA Synthesis
Components:
TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519523
$92.00
10000 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Catalog No. ABIN4219169
$92.21
25 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

EasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA.

Application:
cDNA Synthesis
Components:
EasyScript® RT/RI Enzyme Mix, 2xES Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the, product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519428
$94.00
50 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains EasyScript™ Plus Reverse Transcriptase.
Catalog No. ABIN4219171
$105.15
25 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219165
$118.09
100 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

TransScript First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA.

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519500
$131.00
50 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

From RNA template to cDNA in just 15 minutes completely hassle-free.

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Catalog No. ABIN4219181
$137.50
100 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, gDNA Remover, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 12 kb.
Catalog No. ABIN5519519
$154.00
50 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA.

Application:
cDNA Synthesis
Components:
5xTransScript® All-in-One SuperMix for PCR, 5xTransScript® All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Catalog No. ABIN5519498
$169.00
50 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Catalog No. ABIN4219170
$176.32
100 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219166
$182.79
100 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA.

Application:
cDNA Synthesis
Components:
TransScript® All-in-One SuperMix for PCR, RNase-free Water
Storage:
-20 °C
Specificity:
One-tube format for simple and fast setup and reduce pipetting variability.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 12 kb.
Catalog No. ABIN5519497
$185.00
50 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

From RNA template to cDNA in just 15 minutes completely hassle-free.

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Catalog No. ABIN4219182
$208.68
200 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

One-Step first-strand cDNA synthesis with PCR

Application:
cDNA Synthesis
Components:
EasyScript® One-Step Enzyme Mix, 2x One-Step Reaction Mix, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 4 kb.
Catalog No. ABIN5519430
$211.00
200 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

TransScript® Two-Step RT-PCR SuperMix performs first-strand cDNA synthesis and PCR in two steps.

Application:
cDNA Synthesis
Components:
5xTransScript® All-in-One SuperMix for PCR, 2xTransTaq® HiFi PCR SuperMix II, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 12 kb.
Catalog No. ABIN5519525
$215.00
1 kit
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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