TransScript® II First-Strand cDNA Synthesis SuperMix
cDNA
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- Application
- cDNA Synthesis (cDNA)
- Purpose
- TransScript® II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA.
- Brand
- TransScript®
- Specificity
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Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb. - Characteristics
- The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2xTS II Reaction Mix.
- Components
- 5x TransScript® All-in-One SuperMix for PCR, RNase-free Water
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- Application Notes
- cDNA library construction, 3' and 5' RACE, Multiple copy and low copy gene detection, GC-rich or complex secondary structure RNA template
- Comment
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50 rxnsx20 μl Systems
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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