Micrococcal Nuclease S7
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- Species
- Staphylococcus aureus
- Purpose
- Micrococcal Nuclease S7
- Characteristics
- Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.
- Purification
- Purified by chromatography and assayed by SDS-PAGE.
- Unit Definition
- One Unit causes a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate.
- Biological Activity Comment
- 6,000 units per mg protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Restore with 1 mL deionized water (or equivalent).
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C or at -20° C or colder prior to opening product.
- Expiry Date
- 6 months
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