T4 RNA Ligase 2 (dsRNA Ligase)

Details for Product No. ABIN3188212,
Application
Ligation (Lig)
Options
Characteristics T4 RNA Ligase 2 (dsRNA Ligase) is far more active in joining nicked ends of double stranded RNA over single stranded RNA and requires an adjacent 5´ phosphate and 3´ OH for successful ligation to occur. The enzyme can also ligate dsRNA to dsDNA via the 3´ OH of RNA and 5´ phosphate of DNA to form a double stranded hybrid.
Comment

  • ligate nicks in dsRNA.
  • suitable for ligating the 3' OH terminus of RNA to the 5' phosphate terminus of DNA to form a DNA/RNA hybrid.
Restrictions For Research Use only
Concentration 10 U/μL