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Cas9 Nuclease Protein

GEEN 1000 nM
Catalog No. ABIN3188186
  • Application
    Genome Editing with Engineered Nucleases (GEEN)
    Characteristics
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Guided by a target-specific, single guide RNA (sgRNA), the Cas9 Nuclease Protein serves to unwind the genomic DNA duplex and cleave both strands upon recognition of the target sequence by the sgRNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene.
    Components
    Enzyme supplied with 10X Reaction Buffer
  • Restrictions
    For Research Use only
  • Concentration
    1000 nM
    Buffer
    10 mM Tris-HCl ( pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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