Retroviral Vectors
Retroviral plasmids have been used as vehicles for transferring genes into eukaryotic cells, a process known as transduction. The largest strength of a retroviral vector is in its ability to integrate plasmid DNA with the help of reverse transcriptase into the host genome to generate a cell line with stable expression, rather than transient expression. Unlike transiently expressed genes, integrated, stable expression will persist over multiple generations. Retroviral plasmids can only infect actively dividing cell lines. As a result, cells such as neurons are very resistant to infection and transduction by retroviruses. Furthermore their transfection efficiency is lower than with other viral expression vectors.
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134,801 results:
- Type:
- Empty
- Application:
- Negative Control, Cloning
- Vector type:
- Retroviral Vector
- Vector backbone:
- pRFP-C-RS
- Resistance Gene:
- Chloramphenicol
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- RFP tag
- Expression:
- Transient, Stable
- Type:
- Empty
- Application:
- Negative Control, Cloning
- Vector type:
- Retroviral Vector
- Vector backbone:
- pRS
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Expression:
- Transient, Stable
- Type:
- shRNA
- Application:
- RNA Interference
- Gene:
- LSM5 Homolog, U6 Small Nuclear RNA Associated (S. Cerevisiae) (LSM5)
- Species:
- Rat
- Vector type:
- Retroviral Vector
- Vector backbone:
- pGFP-V-RS
- Resistance Gene:
- Kanamycin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- GFP tag
- Expression:
- Transient, Stable
- Type:
- shRNA
- Application:
- RNA Interference
- Gene:
- Vac14 Homolog (S. Cerevisiae) (VAC14)
- Species:
- Rat
- Vector type:
- Retroviral Vector
- Vector backbone:
- pGFP-V-RS
- Resistance Gene:
- Kanamycin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- GFP tag
- Expression:
- Transient, Stable
- Type:
- shRNA
- Application:
- RNA Interference
- Gene:
- SEC11 Homolog A (S. Cerevisiae) (SEC11A)
- Species:
- Rat
- Vector type:
- Retroviral Vector
- Vector backbone:
- pGFP-V-RS
- Resistance Gene:
- Kanamycin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- GFP tag
- Expression:
- Transient, Stable
- Type:
- shRNA
- Application:
- RNA Interference
- Gene:
- Protein Z, Vitamin K-Dependent Plasma Glycoprotein (PROZ)
- Species:
- Rat
- Vector type:
- Retroviral Vector
- Vector backbone:
- pGFP-V-RS
- Resistance Gene:
- Kanamycin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- GFP tag
- Expression:
- Transient, Stable
- Type:
- shRNA
- Application:
- RNA Interference
- Gene:
- Dpy-30 Homolog (C. Elegans) (DPY30)
- Species:
- Rat
- Vector type:
- Retroviral Vector
- Vector backbone:
- pGFP-V-RS
- Resistance Gene:
- Kanamycin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- GFP tag
- Expression:
- Transient, Stable
- Type:
- shRNA
- Application:
- RNA Interference
- Gene:
- Dihydrouridine Synthase 1-Like (S. Cerevisiae) (DUS1L)
- Species:
- Rat
- Vector type:
- Retroviral Vector
- Vector backbone:
- pGFP-V-RS
- Resistance Gene:
- Kanamycin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- GFP tag
- Expression:
- Transient, Stable
- Type:
- shRNA
- Application:
- RNA Interference
- Gene:
- Crumbs Homolog 1 (Drosophila) (CRB1)
- Species:
- Rat
- Vector type:
- Retroviral Vector
- Vector backbone:
- pGFP-V-RS
- Resistance Gene:
- Kanamycin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- GFP tag
- Expression:
- Transient, Stable
- Type:
- shRNA
- Application:
- RNA Interference
- Gene:
- Exostosin 2 (EXT2)
- Species:
- Rat
- Vector type:
- Retroviral Vector
- Vector backbone:
- pRFP-C-RS
- Resistance Gene:
- Chloramphenicol
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter
- Fusion Tag:
- RFP tag
- Expression:
- Transient, Stable
