DNA / RNA Ligase
DNA Ligase T4
- Storage:
- -20 °C
- Unit Definition:
- One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37° C (Weiss, B., et al., JBC 243, 4543 (1968).
Delivery in 5 to 7 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond by ligating the 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
- Application:
- Ligation
- Components:
- T4 DNA Ligase 250 unit/μl, 10x T4 DNA Ligase Buffer
- Unit Definition:
- One unit of T4 DNA Ligase is defined as the amount of enzyme required to catalyze the ligation of greater than 90% of λ DNA-HindIII fragments in 20 μl at 16°C in 30 minutes. One unit of this enzyme corresponds to 0.008 Weiss units.
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Ligation
- Concentration:
- 1 U/μL
- Components:
- Enzyme supplied with 5X Reaction Buffer
- Storage:
- -20 °C
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
T4 DNA Ligase (5 u/ul)
- Application:
- Ligation
- Concentration:
- 5 U/μL
- Storage:
- -80 °C
- Specificity:
- An enzyme which anneals DNA strands together
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Ligation
- Concentration:
- 10 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of E. coli DNA Ligase that is required to give 50% ligation of HindIII-digested λ DNA in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E.coli DNA Ligase Reaction Buffer (concentration of 5' DNA termini is 0.12 μM [300 μg/ml]).
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end.
- Application:
- Ligation
- Storage:
- -20 °C
Delivery in 11 to 16 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
- Application:
- Ligation
- Components:
- T4 DNA Ligase 250 unit/μl, 10x T4 DNA Ligase Buffer
- Unit Definition:
- One unit of T4 DNA Ligase is defined as the amount of enzyme required to catalyze the ligation of greater than 90% of λ DNA-HindIII fragments in 20 μl at 16°C in 30 minutes. One unit of this enzyme corresponds to 0.008 Weiss units.
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Biotin protein ligase, BirA(His tag)
- Application:
- Ligation
- Components:
- BirA, 10xBirA Buffer A, 10xBirA Buffer B, D-Biotin, manual
- Storage:
- -80 °C
Will be delivered in 31 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Catalyzes the formation of a phosphodiester bond between a pre-adenylated 5' phosphate (DNA or RNA) and the 3' hydroxyl of RNA
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- antibodies-online
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- genomics-online
- genomics-online.com (english)