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E. coli DNA Ligase Enzyme

Lig 10 U/μL
Catalog No. ABIN3188192
$124.56
Plus shipping costs $45.00 and $22.00 dry ice
1000 U
local_shipping Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    Ligation (Lig)
    Characteristics
    E.coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3'-hydroxyl and 5'-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on nicked DNA but is not effective for the formation of DNA-RNA or RNA-RNA hybrids.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of E. coli DNA Ligase that is required to give 50% ligation of HindIII-digested λ DNA in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E.coli DNA Ligase Reaction Buffer (concentration of 5' DNA termini is 0.12 μM [300 μg/ml]).
  • Comment

    • Ligation of dsDNA with cohesive termini
    • cDNA cloning of products from second strand cDNA synthesis experiments
    • Alternative to T4 DNA Ligase when bluntend ligation is not required
    • For ligation of bluntend fragments use T4 DNA Ligase (abm Cat. No. G467)

    Restrictions
    For Research Use only
  • Concentration
    10 U/μL
    Buffer
    50 mM Tris-HCl ( pH 7.5), 1 mM EDTA, 1 mM DTT, 200 mM NaCl, and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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