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Inorganic Pyrophosphatase, Thermostable Enzyme

2 U/μL
Catalog No. ABIN3188197
$59.85
Plus shipping costs $45.00 and $20.00 dry ice
100 μL
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  • Characteristics
    Inorganic pyrophosphatase, Thermostable catalyzes the hydrolysis reaction of inorganic pyrophosphate to orthophosphate. P2O7-4 + H2O -> 2HPO4-2.
    Source: The source of this protein is a recombinant E. coli strain encoding pyrophosphatase from Thermococcus litoralis.
    Unit Definition
    One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
  • Restrictions
    For Research Use only
  • Concentration
    2 U/μL
    Buffer
    20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50 % Glycerol, pH 8.0 at 25 °C.
    Storage
    -20 °C
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