Inorganic pyrophosphatase, Thermostable catalyzes the hydrolysis reaction of inorganic pyrophosphate to orthophosphate. P2O7-4 + H2O -> 2HPO4-2. Source: The source of this protein is a recombinant E. coli strain encoding pyrophosphatase from Thermococcus litoralis.
Unit Definition
One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).