Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
- Application:
- Polymerase Chain Reaction
- Components:
- - 0.1 U/µl Taq DNA Polymerase
- Reaction buffer
- 3 mM MgCl2
- 0.4 mM dNTPs - Storage:
- -20 °C
- Specificity:
- The mixture is optimized for consistent and efficient routine PCR amplifications. It can amplify up to 8 kb fragment from lambda DNA.
Delivery in 6 to 8 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Storage:
- RT
- Harada, Takagi, Nakano, Yamaji, Inui: "Protein arginine methyltransferase 10 is required for androgen-dependent proliferation of LNCaP prostate cancer cells." in: Bioscience, biotechnology, and biochemistry, Vol. 79, Issue 9, pp. 1430-7, 2015 (Pubmed)
- Taboada, Hermida, Pardo, Vera, Piferrer, Viñas, Bouza, Martínez: "Fine mapping and evolution of the major sex determining region in turbot (Scophthalmus maximus)." in: G3 (Bethesda, Md.), Vol. 4, Issue 10, pp. 1871-80, 2014 (Pubmed)
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
- Lee, Rollwagen-Bollens, Bollens, Faber-Hammond: "Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake." in: Ecotoxicology and environmental safety, Vol. 114, Issue , pp. 318-25, 2015 (Pubmed)
- González, Brito, González: "Identification of glycoproteins secreted by wild-type Botrytis cinerea and by protein O-mannosyltransferase mutants." in: BMC microbiology, Vol. 14, Issue , pp. 254, 2015 (Pubmed)
- Boyd, Tennant, Melendez, Toema, Galen, Geddes, Levine: "Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood." in: Journal of applied microbiology, Vol. 118, Issue 5, pp. 1199-209, 2015 (Pubmed)
- Miersch, Li, Hanna, McLaughlin, Hornsby, Matsuguchi, Paduch, Sääf, Wells, Koide, Kossiakoff, Sidhu: "Scalable high throughput selection from phage-displayed synthetic antibody libraries." in: Journal of visualized experiments : JoVE, Vol. , Issue 95, pp. 51492, 2015 (Pubmed)
- Thekke-Veetil, Polashock, Marn, Plesko, Schilder, Keller, Martin, Tzanetakis: "Population structure of blueberry mosaic associated virus: Evidence of reassortment in geographically distinct isolates." in: Virus research, Vol. 201, Issue , pp. 79-84, 2015 (Pubmed)
- Maxfield-Taylor, Mujic, Rao: "First detection of the larval chalkbrood disease pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in adult bumble bees." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124868, 2015 (Pubmed)
- Frías, Brito, González, González: "The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface." in: Molecular plant pathology, Vol. 15, Issue 4, pp. 342-51, 2014 (Pubmed)
- Acanda, Martínez, Prado, González, Rey: "EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine." in: Plant cell reports, Vol. 33, Issue 3, pp. 471-81, 2014 (Pubmed)
- Zhang, Liu, Liu, Zhang, Cao, Ye, Wang, Zhang, Xiao, Yang, Li, Bai: "Transcriptome profiling of a multiple recurrent muscle-invasive urothelial carcinoma of the bladder by deep sequencing." in: PLoS ONE, Vol. 9, Issue 3, pp. e91466, 2014 (Pubmed)
- Boyd, Tennant, Saague, Simon, Muhsen, Ramachandran, Cross, Galen, Pasetti, Levine: "Serum bactericidal assays to evaluate typhoidal and nontyphoidal Salmonella vaccines." in: Clinical and vaccine immunology : CVI, Vol. 21, Issue 5, pp. 712-21, 2014 (Pubmed)
- Show more References
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
- Laurin-Lemay, Angers, Benrey, Brodeur: "Inconsistent genetic structure among members of a multitrophic system: did bruchid parasitoids (Horismenus spp.) escape the effects of bean domestication?" in: Bulletin of entomological research, Vol. 103, Issue 2, pp. 182-92, 2013 (Pubmed)
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Concentration:
- 5 mg/mL
- Storage:
- 4 °C,-20 °C
- Unit Definition:
- One unit equals 0.02 kunitz units and at least 2,000 units are present per mg of protein
Delivery in 1 to 2 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Storage:
- 4 °C,-20 °C
- Unit Definition:
- Deoxyribonuclease I application is one unit will cause an increase in the absorbance at 260 nm of 0.001 per minute per ml at 25° C, pH 5.0 when acting on highly polymerized DNA according to the assay method of Kunitz (J.Gen.Physiol. 33, 349 and 363 (1950)).
Delivery in 1 to 2 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Concentration:
- 0.57 mg/mL
- Storage:
- 4 °C
Delivery in 1 to 2 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Storage:
- -20 °C
- Unit Definition:
- One unit renders acid soluble 1 μg of denatured DNA (ssDNA) per minute at 37° C, pH 4.6.
Delivery in 1 to 2 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Storage:
- 4 °C,-20 °C
Delivery in 1 to 2 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Storage:
- -20 °C
- Unit Definition:
- One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37° C (Weiss, B., et al., JBC 243, 4543 (1968).
Delivery in 1 to 2 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- DNA Modification
- Concentration:
- 20 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Genome Editing with Engineered Nucleases
- Concentration:
- 1000 nM
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Genome Editing with Engineered Nucleases
- Concentration:
- 1000 nM
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Restriction Digest
- Concentration:
- 20000 U/mL
- Components:
- EcoRI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
- Storage:
- -20 °C
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- DNA Amplification
- Concentration:
- 5 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Concentration:
- 2 U/μL
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Restriction Digest
- Concentration:
- 20000 U/mL
- Components:
- KpnI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
- Storage:
- -20 °C
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Ligation
- Concentration:
- 10 U/μL
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Concentration:
- 5 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of Uracil DNA Glycosylase that catalyzes the release of 60 pmol of uracil per one minute from uracil-containing, double-stranded DNA at 37°C.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)