Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
- Application:
- DNA Modification
- Concentration:
- 250 U/μL
- Storage:
- -20 °C
- Specificity:
- One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 ℃, which corresponds approximately to complete digestion of 37 μg DNA. Note that 1 KU=1000 units.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Pfu DNA Polymerase for accurate and consistent performance in PCR.
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2.5 units/μL
- Storage:
- -20 °C
- Specificity:
- An enzyme that catalyzes the polymerization of nucleotides
- Jin, Zhuo, Guo, Field: "p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24698-705, 2005 (Pubmed)
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Pure recombinant active bacterial TurboNuclease
- Application:
- DNA Modification
- Concentration:
- 250 U/μL
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Cost-effective quick PCR with improved fidelity? With Taq Plus DNA Polymerase.
- Application:
- Polymerase Chain Reaction
- Concentration:
- 5 U/μL
- Storage:
- -20 °C
- Specificity:
- With Taq Plus DNA Polymerase, you can expect
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
- Xu, Cheng, Li, Wang, Zhu, Ma, Zhang, Dong, Wang, Zhang: "Identification of substituent groups and related genes involved in salecan biosynthesis in Agrobacterium sp. ZX09." in: Applied microbiology and biotechnology, Vol. 101, Issue 2, pp. 585-598, 2016 (Pubmed)
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase (with 2.5 mM dNTPs)
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Taq DNA Polymerase for PCR and primer extension reactions at elevated temperature.
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2.5 units/μL
- Components:
- Taq DNA Polymerase + 10X Reaction Buffer
- Storage:
- -20 °C
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
- Lee, Rollwagen-Bollens, Bollens, Faber-Hammond: "Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake." in: Ecotoxicology and environmental safety, Vol. 114, Issue , pp. 318-25, 2015 (Pubmed)
- González, Brito, González: "Identification of glycoproteins secreted by wild-type Botrytis cinerea and by protein O-mannosyltransferase mutants." in: BMC microbiology, Vol. 14, Issue , pp. 254, 2015 (Pubmed)
- Boyd, Tennant, Melendez, Toema, Galen, Geddes, Levine: "Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood." in: Journal of applied microbiology, Vol. 118, Issue 5, pp. 1199-209, 2015 (Pubmed)
- Miersch, Li, Hanna, McLaughlin, Hornsby, Matsuguchi, Paduch, Sääf, Wells, Koide, Kossiakoff, Sidhu: "Scalable high throughput selection from phage-displayed synthetic antibody libraries." in: Journal of visualized experiments : JoVE, Vol. , Issue 95, pp. 51492, 2015 (Pubmed)
- Thekke-Veetil, Polashock, Marn, Plesko, Schilder, Keller, Martin, Tzanetakis: "Population structure of blueberry mosaic associated virus: Evidence of reassortment in geographically distinct isolates." in: Virus research, Vol. 201, Issue , pp. 79-84, 2015 (Pubmed)
- Maxfield-Taylor, Mujic, Rao: "First detection of the larval chalkbrood disease pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in adult bumble bees." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124868, 2015 (Pubmed)
- Frías, Brito, González, González: "The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface." in: Molecular plant pathology, Vol. 15, Issue 4, pp. 342-51, 2014 (Pubmed)
- Acanda, Martínez, Prado, González, Rey: "EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine." in: Plant cell reports, Vol. 33, Issue 3, pp. 471-81, 2014 (Pubmed)
- Zhang, Liu, Liu, Zhang, Cao, Ye, Wang, Zhang, Xiao, Yang, Li, Bai: "Transcriptome profiling of a multiple recurrent muscle-invasive urothelial carcinoma of the bladder by deep sequencing." in: PLoS ONE, Vol. 9, Issue 3, pp. e91466, 2014 (Pubmed)
- Boyd, Tennant, Saague, Simon, Muhsen, Ramachandran, Cross, Galen, Pasetti, Levine: "Serum bactericidal assays to evaluate typhoidal and nontyphoidal Salmonella vaccines." in: Clinical and vaccine immunology : CVI, Vol. 21, Issue 5, pp. 712-21, 2014 (Pubmed)
- Show more References
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Problems with non-specific amplification and primer-dimer formation? Try HotStart DNA Polymerase.
- Application:
- Polymerase Chain Reaction, Multiplex Assay
- Storage:
- -20 °C
- Specificity:
- With HotStart DNA Polymerase, you can achieve
- The highest specificity with minimal background
- Superior performance
- Improved yield of desired product
- Paulson, Le, Dickson, Ehlting, von Aderkas, Perlman: "Transcriptome analysis provides insight into venom evolution in a seed-parasitic wasp, Megastigmus spermotrophus." in: Insect molecular biology, Vol. 25, Issue 5, pp. 604-16, 2016 (Pubmed)
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.
- Application:
- cDNA Synthesis
- Components:
- cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
- Storage:
- -20 °C
- Specificity:
- - Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
- Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
- Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.
- Application:
- cDNA Synthesis
- Components:
- cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
- Storage:
- -20 °C
- Specificity:
- - Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains EasyScript™ Plus Reverse Transcriptase.
- Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase (with 2.5 mM dNTPs)
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase (with 2.5 mM dNTPs)
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
- Laurin-Lemay, Angers, Benrey, Brodeur: "Inconsistent genetic structure among members of a multitrophic system: did bruchid parasitoids (Horismenus spp.) escape the effects of bean domestication?" in: Bulletin of entomological research, Vol. 103, Issue 2, pp. 182-92, 2013 (Pubmed)
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.
- Application:
- cDNA Synthesis
- Components:
- cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
- Storage:
- -20 °C
- Specificity:
- - Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
- Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase (with 2.5 mM dNTPs)
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
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- genomics-online.com (english)
