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T4 Polynucleotide Kinase

10 U/μL
Catalog No. ABIN3188238
  • Characteristics
    T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-hydroxyl terminus of double and single-stranded RNA and DNA, oligonucleotides or nucleoside 3´-monophosphates. The enzyme is also capable of catalyzing the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of T4 Polynucleotide Kinase that catalyzes the incorporation of 1 nmol γ-phosphate from ATP to the 5'-hydroxyl termini of micrococcal nuclease-treated DNA in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
  • Comment

    • Labelling 5'termini of DNA or RNA to be used as:
    • primers for DNA sequencing
    • primers for PCR
    • probes for hybridization
    • probes for transcript mapping
    • markers for gel electrophoresis
    • Addition of 5'phosphates to oligonucleotides, PCR products, and DNA or RNA prior to ligation
    • Removal of 3´phosphoryl groups

    Restrictions
    For Research Use only
  • Concentration
    10 U/μL
    Buffer
    10 mM Tris-HCl ( pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 μM ATP, and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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