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Restriction Enzymes

Restriction enzymes (nucleases) are a class of enzymatic proteins that cut nucleotide chains at given locations or specific sequences. Restriction endonucleases are the most common form of restriction enzyme used in molecular cloning. Endonucleases recognize an internal sequence within a nucleotide chain, and cleave at that specific sequence generating either sticky ends (containing a 3’ or 5’ overhang) or blunt (containing no 3’ or 5’ overhang) ends. These ends may then be joined to a compatible end on another sequence via a DNA-ligase to prepare a desired sequence.

Restriction exonucleases do not recognize or cleave internal sequences on DNA, and instead function by removing nucleotides from a “free” or open end. Different exonucleases may work, either in a 5’ -> 3’ direction, or in a 3 -> 5’ direction. In molecular cloning, exonucleases are most commonly used to remove either a 3’ or 5’ overhang from a “sticky end” and leave, instead a “blunt” end (e.g. after a sequence has been cleaved with an endonuclease).

On genomics-online you will find a well selected variety of restriction enzymes. If you have any question regarding our products, please contact our customer support via live chat, phone, or email.

32 results:

EcoRI ClearCut Enzyme

RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188194
$59.85
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 3 to 4 Business Days

KpnI ClearCut Enzyme

RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188199
$59.85
Plus shipping costs $45.00 and $24.00 dry ice
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days

XbaI Enzyme

RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188218
$72.79
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 3 to 4 Business Days
Catalog No. ABIN5519446
$74.36
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519452
$74.36
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519456
$74.36
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days

MluI ClearCut Enzyme

RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188200
$79.26
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 3 to 4 Business Days
Catalog No. ABIN5519448
$81.52
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519454
$81.52
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519458
$81.52
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519460
$81.52
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519472
$81.52
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519474
$81.52
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519476
$81.52
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519480
$81.52
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519464
$84.38
Plus shipping costs $45.00 and $24.00 dry ice
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Delivery in 12 to 16 Business Days
Catalog No. ABIN5519468
$84.38
Plus shipping costs $45.00 and $24.00 dry ice
local_shipping Shipping to: United States
Delivery in 12 to 16 Business Days
Catalog No. ABIN5519478
$84.38
Plus shipping costs $45.00 and $24.00 dry ice
local_shipping Shipping to: United States
Delivery in 12 to 16 Business Days
Catalog No. ABIN5519482
$84.38
Plus shipping costs $45.00 and $24.00 dry ice
local_shipping Shipping to: United States
Delivery in 12 to 16 Business Days
Catalog No. ABIN5519488
$84.38
Plus shipping costs $45.00 and $24.00 dry ice
local_shipping Shipping to: United States
Delivery in 12 to 16 Business Days
  • Type Enzyme
    • Enzyme
  • Application Restriction Digest (RD)
    • Restriction Digest (RD)
    • Polymerase Chain Reaction (PCR)
    • Quantitative real-time PCR (qPCR)
    • Genome Editing with Engineered Nucleases (GEEN)
    • cDNA Synthesis (cDNA)
    • DNA Modification (DNA Mod)
    • Ligation (Lig)
    • DNA Amplification (DNA Amp)
    • RNA Modification (RNA Mod)
    • DNA Manipulation (DNA Man)
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