AflII

Details for Product No. ABIN3188181,
Application
Restriction Digest (RD)
Options
Characteristics An E. coli strain that carries the AflII gene from Anabaena flos-aquae (CCAP 1403/13f). The AflII enzyme recognizes and cuts the sequence C^TTAAG.
Components AflII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Comment

  • Restriction enzyme digestion of doublestranded DNA
  • Reaction: 1X Universal Restriction Enzyme Reaction Buffer
  • Reaction Buffer: Buffer 1: 50%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
  • Heat Inactivation: 65°C for 20 min
  • Methylation Sensitivity:
  • dam methylation: Not Sensitive
  • dcm methylation: Not Sensitive
  • CpG Methylation: Not Sensitive

Restrictions For Research Use only
Concentration 20000 U/mL
Buffer 10 mM Tris-HCl ( pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50 % (v/v) Glycerol
Storage -20 °C