KpnI ClearCut

Details for Product No. ABIN3188199,
Application
Restriction Digest (RD)
Options
Characteristics ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
Components KpnI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Comment

  • Restriction enzyme digestion of doublestranded DNA
  • Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
  • Source: An E. coli strain that carries the KpnI gene from Klebsiella pneumoniae OK8 (ATCC 49790). The KpnI ClearCut enzyme recognizes and cuts the sequence GGTAC^C.
  • Reaction Buffer: Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%
  • Methylation Sensitivity
  • dam methylation: Not Sensitive
  • dcm methylation: Not Sensitive
  • CpG Methylation: Not Sensitive
  • Heat Inactivation: No

Restrictions For Research Use only
Concentration 20000 U/mL
Buffer 10 mM Tris-HCl ( pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50 % (v/v) Glycerol
Storage -20 °C