Lentiviruses are a subclass of Retroviruses. They derived from the human immunodeficiency virus (HIV-1) and became suitable tools for gene delivery in mammalian cells, with most of the viral genes removed. Lentiviral vectors only contain the LTRs and the packaging signal, Ψ. Lentiviral packaging genes are provided on separate plasmids, so the pseudo lentiviral particles are replication deficient. Lentivirus infects both, easy-to-transfect & hard-to-transfect. The advantageous feature of lentivirus vectors is the ability to mediate potent transduction and stable expression into dividing and non-dividing cells both in vitro and in vivo.
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