NotI ClearCut

Details for Product No. ABIN3188202,
Application
Restriction Digest (RD)
Options
Characteristics ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
Components NotI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Comment

  • Restriction enzyme digestion of doublestranded DNA
  • Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
  • Source: An E. coli strain that carries the modified NotI gene from Nocardia otitidiscaviarum (ATCC 14630). The NotI ClearCut enzyme recognizes and cuts the sequence GC^GGCCGC.
  • Reaction Buffer: Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 25%, Universal Buffer: 100%
  • Methylation Sensitivity
  • dam methylation: Not Sensitive
  • dcm methylation: Not Sensitive
  • CpG Methylation: Blocked
  • Heat Inactivation: 65°C for 20 min

Restrictions For Research Use only
Concentration 20000 U/mL
Buffer 10 mM Tris-HCl ( pH 7.5), 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15 % Triton® X-100, and 50 % (v/v) Glycerol
Storage -20 °C