NotI ClearCut

Details for Product No. ABIN3188202, Supplier: Log in to see
Restriction Digest (RD)
Log in to see
Supplier Product No.
Log in to see
Characteristics ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
Components NotI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer

  • Restriction enzyme digestion of doublestranded DNA
  • Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
  • Source: An E. coli strain that carries the modified NotI gene from Nocardia otitidiscaviarum (ATCC 14630). The NotI ClearCut enzyme recognizes and cuts the sequence GC^GGCCGC.
  • Reaction Buffer: Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 25%, Universal Buffer: 100%
  • Methylation Sensitivity
  • dam methylation: Not Sensitive
  • dcm methylation: Not Sensitive
  • CpG Methylation: Blocked
  • Heat Inactivation: 65°C for 20 min

Restrictions For Research Use only
Concentration 20000 U/mL
Buffer 10 mM Tris-HCl ( pH 7.5), 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15 % Triton® X-100, and 50 % (v/v) Glycerol
Storage -20 °C