DNA Ligase T4

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Purpose DNA Ligase T4
Biological Activity Comment 6.0 units/μL
Unit Definition One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37° C (Weiss, B., et al., JBC 243, 4543 (1968).
Application Notes

Application Note: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP,  pH 7.5 @ 25 °C.

Comment

Synonyms: DNA Ligase T4

Restrictions For Research Use only
Format Liquid
Buffer

Buffer: See application note.

Storage -20 °C