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RNase III, E. coli

RNA Mod 1 U/μL
Catalog No. ABIN3188207
  • Application
    RNA Modification (RNA Mod)
    Characteristics
    RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3´-overhangs with 5' phosphate and 3' hydroxyl termini.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.
  • Comment

    • dsRNA digestion to short dsRNA fragments
    • Useful for RNA processing, maturation and structure studies
    • Directed cleavage of RNA
    • siRNA preparation for RNA interference in mammalian cells

    Restrictions
    For Research Use only
  • Concentration
    1 U/μL
    Buffer
    50 mM Tris-HCl ( pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1 % Triton® X-100 and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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