RNase III, E. coli
RNA Mod
1 U/μL
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- Application
- RNA Modification (RNA Mod)
- Characteristics
- RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3´-overhangs with 5' phosphate and 3' hydroxyl termini.
- Components
- Enzyme supplied with 10X Reaction Buffer
- Unit Definition
- One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.
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- Comment
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- dsRNA digestion to short dsRNA fragments
- Useful for RNA processing, maturation and structure studies
- Directed cleavage of RNA
- siRNA preparation for RNA interference in mammalian cells
- Restrictions
- For Research Use only
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- Concentration
- 1 U/μL
- Buffer
- 50 mM Tris-HCl ( pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1 % Triton® X-100 and 50 % (v/v) Glycerol.
- Storage
- -20 °C
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