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FlyCut™ EcoRI

RD
Catalog No. ABIN5519452
  • Application
    Restriction Digest (RD)
    Purpose
    Super fast restriction enzyme
    Brand
    FlyCut™
    Specificity
    5'...G^AATTC...3'
    3'...CTTAA^G...5'
    Characteristics
    FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.
    Components
    Enzyme, 10X Buffer, 10X DNA Loading Buffer
    Unit Definition
    One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
  • Application Notes
    Genomic DNA, plasmid DNA, PCR product
    Comment

    Fast: 5 minutes DNA digestion.
    Simple: universal buffer for all restriction enzymes.

    Restrictions
    For Research Use only
  • Buffer
    Storage Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/mL BSA, 50 % Glycerol
    10xFlyCut® Buffer: 500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/mL BSA
    Storage
    -20 °C
    Storage Comment
    at -20°C for two years
    Expiry Date
    24 months
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