EcoRI ClearCut

Details for Product No. ABIN3188194,
Application
Restriction Digest (RD)
Options
Characteristics ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
Components EcoRI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Comment

  • Restriction enzyme digestion of doublestranded DNA ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 515 minutes).
  • Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
  • Source: An E. coli strain that carries the modified EcoRI gene from E. coli RY13. The EcoRI enzyme recognizes and cuts the sequence G^AATTC.
  • Reaction Buffer: Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
  • Heat Inactivation:65°C for 20 min
  • Methylation Sensitivity:
  • dam methylation: Not Sensitive
  • dcm methylation: Not Sensitive
  • CpG Methylation: Blocked by Some Combinations of Overlapping

Restrictions For Research Use only
Concentration 20000 U/mL
Storage -20 °C