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EcoRI ClearCut Enzyme

RD 20000 U/mL
Catalog No. ABIN3188194
$59.85
Plus shipping costs $45.00 and $24.00 dry ice
20000 U
local_shipping Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    Restriction Digest (RD)
    Characteristics
    ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
    Components
    EcoRI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
  • Comment

    • Restriction enzyme digestion of doublestranded DNA ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 515 minutes).
    • Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
    • Source: An E. coli strain that carries the modified EcoRI gene from E. coli RY13. The EcoRI enzyme recognizes and cuts the sequence G^AATTC.
    • Reaction Buffer: Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
    • Heat Inactivation:65°C for 20 min
    • Methylation Sensitivity:
    • dam methylation: Not Sensitive
    • dcm methylation: Not Sensitive
    • CpG Methylation: Blocked by Some Combinations of Overlapping

    Restrictions
    For Research Use only
  • Concentration
    20000 U/mL
    Storage
    -20 °C
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