Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransFast® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
Delivery in 6 to 7 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransTaq®-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...A^GATCT...3'
3'...TCTAG^A...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
- Storage:
- -20 °C
- Unit Definition:
- One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...C^CATGG...3'
3'...GGTAC^C...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...CCGC^GG...3'
3'...GG^CGCC...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...GC^GGCCGC...3'
3'...CGCCGG^CG...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransTaq®-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransFast® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...C^CCGGG...3'
3'...GGGCC^C...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...C^GGCGG...3'
3'...GGCGG^C...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...G^CTAGC...3'
3'...CGATC^G...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
ready-to-use Pfu PCR SuperMix
- Application:
- Polymerase Chain Reaction
- Components:
- 2xEasyPfu PCR SuperMix (-dye ), ddH2O
- Storage:
- -20 °C
- Specificity:
- EasyPfu PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 0.5 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 6 kb.
Amplification of plasmid DNA fragment up to 10 kb.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
suitable for high fidelity TA cloning
- Application:
- Polymerase Chain Reaction
- Components:
- 2xTransTaq®-T PCR SuperMix (-dye), ddH2O
- Storage:
- -20 °C
- Specificity:
- TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
suitable for high fidelity TA cloning
- Application:
- Polymerase Chain Reaction
- Components:
- 2xTransTaq®-T PCR SuperMix (+dye), ddH2O
- Storage:
- -20 °C
- Specificity:
- TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Components:
- - 0.1 U/µl Taq DNA Polymerase
- Reaction buffer
- 3 mM MgCl2
- 0.4 mM dNTPs - Storage:
- -20 °C
- Specificity:
- The mixture is optimized for consistent and efficient routine PCR amplifications. It can amplify up to 8 kb fragment from lambda DNA.
Delivery in 6 to 8 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
ready-to-use mixture for the amplification of genomic DNA
- Application:
- Polymerase Chain Reaction
- Components:
- 2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (+dye ), ddH2O
- Storage:
- -20 °C
- Specificity:
- TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
ready-to-use mixture for the amplification of genomic DNA
- Application:
- Polymerase Chain Reaction
- Components:
- 2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (-dye ), ddH2O
- Storage:
- -20 °C
- Specificity:
- TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...C^CATGG...3'
3'...GGATC^C...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
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