Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
From RNA template to cDNA in just 15 minutes completely hassle-free.
- Application:
- cDNA Synthesis
- Storage:
- -20 °C
- Specificity:
- - Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
- Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
- Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
- Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
- Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
- Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
- Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
- Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
- Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
- Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
- Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
- Show more References
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Concentration:
- 5 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of Uracil DNA Glycosylase that catalyzes the release of 60 pmol of uracil per one minute from uracil-containing, double-stranded DNA at 37°C.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Restriction Digest
- Concentration:
- 20000 U/mL
- Components:
- EcoRI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
- Storage:
- -20 °C
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Restriction Digest
- Concentration:
- 20000 U/mL
- Components:
- KpnI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
- Storage:
- -20 °C
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Short on time? TaqFast DNA Polymerase to the rescue.
- Application:
- Polymerase Chain Reaction
- Concentration:
- 5 U/μL
- Storage:
- -20 °C
- Specificity:
- With TaqFast DNA Polymerase, you can expect:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80 %
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Cost-effective quick PCR with improved fidelity? With Taq Plus DNA Polymerase.
- Application:
- Polymerase Chain Reaction
- Concentration:
- 5 U/μL
- Storage:
- -20 °C
- Specificity:
- With Taq Plus DNA Polymerase, you can expect
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
- Xu, Cheng, Li, Wang, Zhu, Ma, Zhang, Dong, Wang, Zhang: "Identification of substituent groups and related genes involved in salecan biosynthesis in Agrobacterium sp. ZX09." in: Applied microbiology and biotechnology, Vol. 101, Issue 2, pp. 585-598, 2016 (Pubmed)
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Concentration:
- 2 U/μL
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
High Efficiency Taq Mastermix
- Application:
- Polymerase Chain Reaction
- Components:
- 2xEasyTaq® PCR SuperMix ( -dye ), ddH2O
- Storage:
- -20 °C
- Specificity:
- Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 4 kb.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Struggling to amplify long targets? Long-Range DNA Polymerase will end your fight.
- Application:
- Polymerase Chain Reaction
- Concentration:
- 5 U/μL
- Storage:
- -20 °C
- Specificity:
- With Long-Range DNA Polymerase, you can expect:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Efficient amplification of DNA templates up to 20kb
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Proteinase K is commonly used in molecular biology to remove protein contamination.
- Application:
- DNA Isolation, Endotoxin Removal, Immunohistochemistry
- Storage:
- 4 °C
- Unit Definition:
- One unit is defined as the enzyme activity that produce1 µmol of tyrosine per minute from casein at 37°C at pH 7.5. Refer to the certificate of analysis for specific values for the present lot.
- Specificity:
- DNase is not detected in quality control procedure of incubation 40 µg Proteinase K with 1 µg λ DNA for 6 hours at 37°C.
RNase is not detected in quality control procedure of incubation 40 µg Proteinase K with 2 µg RNA for 2 hours at 37°C. This preparation is considered as RNase and DNase free.
Each lot was tested to ensure the absence of Nucleases and DNA.
Delivery in 11 to 13 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Restriction Digest
- Concentration:
- 20000 U/mL
- Components:
- XbaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
- Storage:
- -20 °C
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.
- Application:
- cDNA Synthesis
- Concentration:
- 200 U/μL
- Storage:
- -20 °C
- Specificity:
- EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- DNA Modification
- Concentration:
- 100 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of Exonuclease III, E. coli that is required to catalyze the release of 1 nmol of acid soluble nucleotides from double stranded DNA in 30 minutes at 37°C in 1X Exonuclease III Reaction Buffer.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Problems with non-specific amplification and primer-dimer formation? Try HotStart DNA Polymerase.
- Application:
- Polymerase Chain Reaction, Multiplex Assay
- Storage:
- -20 °C
- Specificity:
- With HotStart DNA Polymerase, you can achieve
- The highest specificity with minimal background
- Superior performance
- Improved yield of desired product
- Paulson, Le, Dickson, Ehlting, von Aderkas, Perlman: "Transcriptome analysis provides insight into venom evolution in a seed-parasitic wasp, Megastigmus spermotrophus." in: Insect molecular biology, Vol. 25, Issue 5, pp. 604-16, 2016 (Pubmed)
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...G^GATCC...3'
3'...CCTAG^G...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...G^AATTC...3'
3'...CTTAA^G...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Super fast restriction enzyme
- Application:
- Restriction Digest
- Components:
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
- Specificity:
- 5'...A^AGCTT...3'
3'...TTCGA^A...5'
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
The Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.
- Storage:
- -20 °C,-80 °C
- Unit Definition:
- One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransFast® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity.
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
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