Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

368 Products
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    Enzyme
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TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519568
$114.14
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519571
$117.06
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219165
$118.09
100 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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T4 DNA Ligase (5 u/ul)

Application:
Ligation
Concentration:
5 U/μL
Storage:
-80 °C
Specificity:
An enzyme which anneals DNA strands together
Catalog No. ABIN411759
$120.00
250 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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ready-to-use Pfu PCR SuperMix with higher fidelity and yield

Application:
Polymerase Chain Reaction
Components:
2xTransStart® FastPfu PCR SuperMix (-dye ), ddH2O
Storage:
-20 °C
Specificity:
TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 2-4 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 15 kb.
Amplification of plasmid DNA fragment up to 20 kb.
Catalog No. ABIN5519412
$122.89
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Catalog No. ABIN5519440
$122.89
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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FlashPfu is a DNA polymerase for high-fidelity PCR. By fusing Pfu with a high affinity double-stranded DNA binding domain, we have dramatically increased its processivity and extension rate. FlashPfu DNA polymerase is 50-fold more accurate than Taq polymerase and 6-fold more accurate than Pfu polymerase.

Application:
Polymerase Chain Reaction
Components:
FlashPfu DNA Polymerase 1 U/μl, 2x flashPfu PCR Buffer, 25 mM MgSO4
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666438
$124.00
100 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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Application:
Ligation
Concentration:
10 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of E. coli DNA Ligase that is required to give 50% ligation of HindIII-digested λ DNA in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E.coli DNA Ligase Reaction Buffer (concentration of 5' DNA termini is 0.12 μM [300 μg/ml]).
Catalog No. ABIN3188192
$124.56
1000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
SalI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188208
$124.56
10000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Catalog No. ABIN5519577
$125.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 6X DNA Loading Buffer, 50 mM MgSO2
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519545
$125.80
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519548
$128.72
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Rnase A

Application:
RNA Modification
Catalog No. ABIN2953155
$180.90
25 mg
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days
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The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

Application:
Polymerase Chain Reaction
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952618
$180.90
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days
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TransStart® FastPfu Fly PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu Fly DNA polymerase, dNTPs, and optimized buffer.

Application:
Polymerase Chain Reaction
Components:
2xTransStart FastPfu Fly PCR SuperMix, ddH2O
Storage:
-20 °C
Specificity:
TransStart® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 2-6 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 15 kb.
Amplification of plasmid DNA fragment up to 20 kb.
Catalog No. ABIN5519410
$130.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Pfu Buffer, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Catalog No. ABIN5519527
$130.17
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Catalog No. ABIN5519530
$133.09
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.

Application:
cDNA Synthesis
Components:
TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519523
$137.46
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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From RNA template to cDNA in just 15 minutes completely hassle-free.

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Publications
  • Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
  • Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
  • Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
  • Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
  • Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219181
$137.50
100 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
RNA Modification
Concentration:
1 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.
Catalog No. ABIN3188207
$137.50
100 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days