Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

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TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransFast® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity.
Catalog No. ABIN5519492
$82.07
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...GAT^ATC...3'
3'...CTA^TAG...5'
Catalog No. ABIN5519454
$82.07
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...GGTAC^C...3'
3'...C^CATGG...5'
Catalog No. ABIN5519458
$82.07
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...AGT^ACT...3'
3'...TCA^TGA...5'
Catalog No. ABIN5519478
$84.99
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...G^CTAGC...3'
3'...CGATC^G...5'
Catalog No. ABIN5519464
$84.99
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...A^GATCT...3'
3'...TCTAG^A...5'
Catalog No. ABIN5519468
$84.99
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...GCATG^C...3'
3'...C^GTACG...5'
Catalog No. ABIN5519482
$84.99
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^CCGGG...3'
3'...GGGCC^C...5'
Catalog No. ABIN5519488
$84.99
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Application:
Ligation
Components:
T4 DNA Ligase 250 unit/μl, 10x T4 DNA Ligase Buffer
Unit Definition:
One unit of T4 DNA Ligase is defined as the amount of enzyme required to catalyze the ligation of greater than 90% of λ DNA-HindIII fragments in 20 μl at 16°C in 30 minutes. One unit of this enzyme corresponds to 0.008 Weiss units.
Catalog No. ABIN5666454
$85.00
25000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188231
$85.74
500 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Concentration:
10 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of T4 Polynucleotide Kinase that catalyzes the incorporation of 1 nmol γ-phosphate from ATP to the 5'-hydroxyl termini of micrococcal nuclease-treated DNA in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
Catalog No. ABIN3188238
$85.74
1000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
1 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
Catalog No. ABIN3188236
$85.74
100 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Klenow Fragment (3'→5' Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188198
$85.74
500 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
RNA Modification
Concentration:
50 U/μL
Components:
10x Rnase I Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit of the enzyme catalyzes degradation of 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C under standard conditions.
Catalog No. ABIN3188206
$85.74
100 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^TCGAG...3'
3'...GAGCT^C...5'
Catalog No. ABIN5519486
$86.44
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^GGCGG...3'
3'...GGCGG^C...5'
Catalog No. ABIN5519450
$86.44
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...T^CTAGA...3'
3'...AGATC^T...5'
Catalog No. ABIN5519484
$86.44
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...GC^GGCCGC...3'
3'...CGCCGG^CG...5'
Catalog No. ABIN5519466
$87.90
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...CA^TATG...3'
3'...GTAT^AC...5'
Catalog No. ABIN5519462
$87.90
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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suitable for high fidelity TA cloning

Application:
Polymerase Chain Reaction
Components:
2xTransTaq®-T PCR SuperMix (-dye), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Catalog No. ABIN5519420
$89.36
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days