Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

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    Enzyme
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...CGAT^CG...3'
3'...GC^TAGC...5'
Catalog No. ABIN5519470
$55.00
250 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519554
$55.00
250 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519557
$58.00
250 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188193
$59.85
500 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Concentration:
2 U/μL
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Catalog No. ABIN3188197
€52.96
100 μL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
RNA Modification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).
Catalog No. ABIN3188205
€52.96
500 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
Ligation
Concentration:
10 U/μL
Catalog No. ABIN3188211
€52.96
100 μL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Concentration:
2 mg/mL
Components:
Protein supplied with 10X Reaction Buffer.
Storage:
-20 °C
Catalog No. ABIN3188204
€52.96
100 μL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Uracil DNA Glycosylase that catalyzes the release of 60 pmol of uracil per one minute from uracil-containing, double-stranded DNA at 37°C.
Catalog No. ABIN3188217
€52.96
1000 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Cost-effective quick PCR with improved fidelity? With Taq Plus DNA Polymerase.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Taq Plus DNA Polymerase, you can expect
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
Catalog No. ABIN4219099
€52.96
250 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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From RNA template to cDNA in just 15 minutes completely hassle-free.

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Catalog No. ABIN4219180
€52.96
25 reactions
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
EcoRI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188194
€52.96
20000 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
KpnI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188199
€52.96
20000 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Short on time? TaqFast DNA Polymerase to the rescue.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With TaqFast DNA Polymerase, you can expect:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80 %
Catalog No. ABIN4219104
€52.96
250 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Application:
Ligation
Components:
T4 DNA Ligase 250 unit/μl, 10x T4 DNA Ligase Buffer
Unit Definition:
One unit of T4 DNA Ligase is defined as the amount of enzyme required to catalyze the ligation of greater than 90% of λ DNA-HindIII fragments in 20 μl at 16°C in 30 minutes. One unit of this enzyme corresponds to 0.008 Weiss units.
Catalog No. ABIN5666454
€53.10
25000 U
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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A ready-to-use qPCR cocktail with unique hot start reagents

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
A combination of chemical blocking technique with TransStart® hot start technique to achieve complete blocking. Compared with double blocking TransStart® TopTaq, this method provides higher sensitivity, higher specificity, better amplification.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519560
€54.87
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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A ready-to-use qPCR cocktail containing all components except primer and template

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® TopTaq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519564
€54.87
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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Bst DNA Polymerase, Large Fragment is a portion of DNA polymerase of Bacillus stearothermophilus, which catalyzes 5'→3' synthesis of DNA and strand displacement, but lacks 5'→3' and 3'→5' exonuclease activities.

Application:
Polymerase Chain Reaction
Components:
Bst DNA Polymerase, Large Fragment 8.0 unit/μl, 10x BstPol Reaction Buffer, 100 mM MgSO4
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
Catalog No. ABIN5666435
€57.52
2000 U
Plus shipping costs €45.00
Will be delivered in 31 Business Days
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Struggling to amplify long targets? Long-Range DNA Polymerase will end your fight.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Long-Range DNA Polymerase, you can expect:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Efficient amplification of DNA templates up to 20kb
Catalog No. ABIN4219108
€58.69
250 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519568
€60.18
250 units
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days