TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase. Extension rate is about 1-2 kb/min. Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors. Amplification of genomic DNA fragment up to 8 kb.
Characteristics
TransTaq®-T PCR SuperMix is a ready-to-use mixture of TransTaq®-T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2x concentration and used at 1x concentration by adding template, primers and H2O. Efficiency of PCR products with "A" is equal to EasyTaq® DNA polymerase. It is more suitable for high fidelity TA cloning.