Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

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TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq®-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.
Catalog No. ABIN5519583
$32.00
250 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransFast® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity.
Catalog No. ABIN5519492
$34.00
500 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^CCGGG...3'
3'...GGGCC^C...5'
Catalog No. ABIN5519488
$34.00
250 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^GGCGG...3'
3'...GGCGG^C...5'
Catalog No. ABIN5519450
$35.00
250 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...G^CTAGC...3'
3'...CGATC^G...5'
Catalog No. ABIN5519464
€30.97
500 units
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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ready-to-use Pfu PCR SuperMix

Application:
Polymerase Chain Reaction
Components:
2xEasyPfu PCR SuperMix (-dye ), ddH2O
Storage:
-20 °C
Specificity:
EasyPfu PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 0.5 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 6 kb.
Amplification of plasmid DNA fragment up to 10 kb.
Catalog No. ABIN5519398
€37.17
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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suitable for high fidelity TA cloning

Application:
Polymerase Chain Reaction
Components:
2xTransTaq®-T PCR SuperMix (-dye), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Catalog No. ABIN5519420
€37.17
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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suitable for high fidelity TA cloning

Application:
Polymerase Chain Reaction
Components:
2xTransTaq®-T PCR SuperMix (+dye), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Catalog No. ABIN5519418
€37.17
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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ready-to-use mixture for the amplification of genomic DNA

Application:
Polymerase Chain Reaction
Components:
2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (+dye ), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb.
Catalog No. ABIN5519416
€38.05
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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ready-to-use mixture for the amplification of genomic DNA

Application:
Polymerase Chain Reaction
Components:
2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (-dye ), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb.
Catalog No. ABIN5519414
€38.05
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^CATGG...3'
3'...GGATC^C...5'
Catalog No. ABIN5519444
€38.05
50 units
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Catalog No. ABIN5519574
€38.05
250 units
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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Extraction-free DNA amplification from whole blood samples with Bloodirect DNA Polymerase.

Application:
Polymerase Chain Reaction
Concentration:
1 U/μL
Storage:
-20 °C
Specificity:
With Bloodirect DNA Polymerase, you can :
- Eliminate lengthy DNA purification steps
- Achieve direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin
Catalog No. ABIN4219173
€38.08
100 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Catalog No. ABIN5519577
€40.71
250 units
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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Application:
DNA Modification
Concentration:
20 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
Catalog No. ABIN3188195
€41.51
2000 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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The Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.

Storage:
-20 °C,-80 °C
Unit Definition:
One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Catalog No. ABIN5666461
€44.25
500 U
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219164
€47.24
25 reactions
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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TransStart® Green qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except primer and template.

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519539
€47.79
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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A ready-to-use qPCR cocktail containing all components, except probe, primer and template.

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519551
€47.79
1 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.

Application:
cDNA Synthesis
Components:
EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519431
€48.67
10000 units
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days