Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

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    Enzyme
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Supplier: Log in to see

TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Catalog No. ABIN5519574
$43.00
250 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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Extraction-free DNA amplification from whole blood samples with Bloodirect DNA Polymerase.

Application:
Polymerase Chain Reaction
Concentration:
1 U/μL
Storage:
-20 °C
Specificity:
With Bloodirect DNA Polymerase, you can :
- Eliminate lengthy DNA purification steps
- Achieve direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin
Catalog No. ABIN4219173
$43.03
100 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Catalog No. ABIN5519577
$46.00
250 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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Application:
DNA Modification
Concentration:
20 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
Catalog No. ABIN3188195
$46.91
2000 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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The Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.

Storage:
-20 °C,-80 °C
Unit Definition:
One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Catalog No. ABIN5666461
$50.00
500 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219164
$53.38
25 reactions
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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TransStart® Green qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except primer and template.

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519539
$54.00
1 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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A ready-to-use qPCR cocktail containing all components, except probe, primer and template.

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519551
$54.00
1 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.

Application:
cDNA Synthesis
Components:
EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519431
$55.00
10000 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...CGAT^CG...3'
3'...GC^TAGC...5'
Catalog No. ABIN5519470
$55.00
250 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519554
$55.00
250 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519557
$58.00
250 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188193
$59.85
500 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Concentration:
2 U/μL
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Catalog No. ABIN3188197
$59.85
100 μL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
RNA Modification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).
Catalog No. ABIN3188205
$59.85
500 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
Ligation
Concentration:
10 U/μL
Catalog No. ABIN3188211
$59.85
100 μL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Concentration:
2 mg/mL
Components:
Protein supplied with 10X Reaction Buffer.
Storage:
-20 °C
Catalog No. ABIN3188204
$59.85
100 μL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Uracil DNA Glycosylase that catalyzes the release of 60 pmol of uracil per one minute from uracil-containing, double-stranded DNA at 37°C.
Catalog No. ABIN3188217
$59.85
1000 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
EcoRI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188194
$59.85
20000 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
KpnI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188199
$59.85
20000 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days