Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

258 Products
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Type
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    Enzyme
Gene
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Species
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  • 2
  • 1
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Host
  • 15
Application
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Protein Type
  • 9
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Deoxyribonuclease I (DNase I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5’ phosphate.

Application:
DNA Modification
Storage:
-20 °C
Specificity:
32 kDa (monomer)
Catalog No. ABIN2953735
$175.00
1500 units
Plus shipping costs $45.00 Will be delivered in 11 to 16 Business Days
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Application:
Polymerase Chain Reaction
Catalog No. ABIN2190160
$303.90
100 units
Plus shipping costs $45.00 Will be delivered in 6 to 11 Business Days
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Red Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Red Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
Red Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188327
$103.95
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...AGT^ACT...3'
3'...TCA^TGA...5'
Catalog No. ABIN5519478
$8.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...T^CTAGA...3'
3'...AGATC^T...5'
Catalog No. ABIN5519484
$8.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...A^GATCT...3'
3'...TCTAG^A...5'
Catalog No. ABIN5519476
$9.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...G^AATTC...3'
3'...CTTAA^G...5'
Catalog No. ABIN5519452
$10.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...A^GATCT...3'
3'...TCTAG^A...5'
Catalog No. ABIN5519468
$12.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...G^GATCC...3'
3'...CCTAG^G...5'
Catalog No. ABIN5519446
$14.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...GAGCT^C...3'
3'...C^TCGAG...5'
Catalog No. ABIN5519472
$15.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...CCC^GGG...3'
3'...GGG^CCC...5'
Catalog No. ABIN5519480
$15.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...CA^TATG...3'
3'...GTAT^AC...5'
Catalog No. ABIN5519462
$16.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...GAT^ATC...3'
3'...CTA^TAG...5'
Catalog No. ABIN5519454
$17.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^TCGAG...3'
3'...GAGCT^C...5'
Catalog No. ABIN5519486
$19.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...GCATG^C...3'
3'...C^GTACG...5'
Catalog No. ABIN5519482
$21.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...A^AGCTT...3'
3'...TTCGA^A...5'
Catalog No. ABIN5519456
$23.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.
Catalog No. ABIN5519437
$23.75
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...GGTAC^C...3'
3'...C^CATGG...5'
Catalog No. ABIN5519458
$28.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.
Catalog No. ABIN5519425
$31.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...A^GATCT...3'
3'...TCTAG^A...5'
Catalog No. ABIN5519448
$31.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days