Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

367 Products
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    Enzyme
Gene
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Species
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Supplier: Log in to see

ready-to-use Pfu PCR SuperMix with higher fidelity and yield

Application:
Polymerase Chain Reaction
Components:
2xTransStart® FastPfu PCR SuperMix (-dye ), ddH2O
Storage:
-20 °C
Specificity:
TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 2-4 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 15 kb.
Amplification of plasmid DNA fragment up to 20 kb.
Catalog No. ABIN5519412
$77.00
1 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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A ready-to-use qPCR cocktail with UDG for the treatment of DNA contamination

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
UDG and dUTP avoid cross contamination.
Catalog No. ABIN5519542
$77.00
1 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
MluI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188200
$79.26
10000 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
NotI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188202
$79.26
10000 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
4 °C/-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219145
$79.26
5 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Bestaq™ DNA Polymerase is the new standard.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Bestaq™ DNA Polymerase, you can expect
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Catalog No. ABIN4219106
$79.26
250 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
4 °C/-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219144
$79.26
5 mL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Kodaq DNA Polymerase is the solution for a robust PCR system with high fidelity and yield.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Catalog No. ABIN4219111
$79.26
250 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 6X DNA Loading Buffer, 50 mM MgSO2
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519545
$80.00
100 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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The Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.

Storage:
-20 °C,-80 °C
Unit Definition:
One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Catalog No. ABIN5666462
$80.00
1000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519548
$83.00
100 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Pfu Buffer, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Catalog No. ABIN5519527
$85.00
250 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Klenow Fragment (3'→5' Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188198
$85.74
500 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188231
$85.74
500 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
1 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
Catalog No. ABIN3188236
$85.74
100 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
RNA Modification
Concentration:
50 U/μL
Components:
10x Rnase I Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit of the enzyme catalyzes degradation of 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C under standard conditions.
Catalog No. ABIN3188206
$85.74
100 μL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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Concentration:
10 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of T4 Polynucleotide Kinase that catalyzes the incorporation of 1 nmol γ-phosphate from ATP to the 5'-hydroxyl termini of micrococcal nuclease-treated DNA in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
Catalog No. ABIN3188238
$85.74
1000 U
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 3 to 4 Business Days
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E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).

Application:
Quantitative real-time PCR
Catalog No. ABIN3188317
$86.25
200 μL
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 6 to 7 Business Days
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TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Catalog No. ABIN5519530
$88.00
250 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days
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TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.

Application:
cDNA Synthesis
Components:
TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519523
$92.00
10000 units
Plus shipping costs $45.00 and $23.00 dry ice
Delivery in 15 to 19 Business Days