Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

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Proteinase K is commonly used in molecular biology to remove protein contamination.

Application:
DNA Isolation, Endotoxin Removal, Immunohistochemistry
Storage:
4 °C
Unit Definition:
One unit is defined as the enzyme activity that produce1 µmol of tyrosine per minute from casein at 37°C at pH 7.5. Refer to the certificate of analysis for specific values for the present lot.
Specificity:
DNase is not detected in quality control procedure of incubation 40 µg Proteinase K with 1 µg λ DNA for 6 hours at 37°C.
RNase is not detected in quality control procedure of incubation 40 µg Proteinase K with 2 µg RNA for 2 hours at 37°C. This preparation is considered as RNase and DNase free.
Each lot was tested to ensure the absence of Nucleases and DNA.
Catalog No. ABIN6383954
$69.00
100 mg
Plus shipping costs $45.00
Delivery in 11 to 13 Business Days
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M-MLV Reverse Transcriptase (RNaseH-) is a recombinant reverse transcriptase expressed and purified from E. Coil. This enzyme can be used in cDNA synthesis with long messenger RNA templates due to the lacking of RNase H activity by point mutation.

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666450
$69.00
5000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519571
$71.00
250 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
XbaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188218
$72.79
20000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
DNA Modification
Concentration:
100 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Exonuclease III, E. coli that is required to catalyze the release of 1 nmol of acid soluble nucleotides from double stranded DNA in 30 minutes at 37°C in 1X Exonuclease III Reaction Buffer.
Catalog No. ABIN3188232
$72.79
10000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Problems with non-specific amplification and primer-dimer formation? Try HotStart DNA Polymerase.

Application:
Polymerase Chain Reaction, Multiplex Assay
Storage:
-20 °C
Specificity:
With HotStart DNA Polymerase, you can achieve
- The highest specificity with minimal background
- Superior performance
- Improved yield of desired product
Catalog No. ABIN4219098
$72.79
250 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219163
$72.79
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Catalog No. ABIN5519440
$73.15
2500 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Taq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

Application:
Polymerase Chain Reaction
Components:
Taq DNA Polymerase 5 U/μl, 10xTaq PCR Buffer, 25 mM MgCl2
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666456
$75.00
500 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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ready-to-use Pfu PCR SuperMix with higher fidelity and yield

Application:
Polymerase Chain Reaction
Components:
2xTransStart® FastPfu PCR SuperMix (-dye ), ddH2O
Storage:
-20 °C
Specificity:
TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 2-4 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 15 kb.
Amplification of plasmid DNA fragment up to 20 kb.
Catalog No. ABIN5519412
$77.00
1 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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A ready-to-use qPCR cocktail with UDG for the treatment of DNA contamination

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
UDG and dUTP avoid cross contamination.
Catalog No. ABIN5519542
$77.00
1 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
MluI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188200
$79.26
10000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
NotI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188202
$79.26
10000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
4 °C/-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219145
$79.26
5 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Bestaq™ DNA Polymerase is the new standard.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Bestaq™ DNA Polymerase, you can expect
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Catalog No. ABIN4219106
$79.26
250 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
4 °C/-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219144
$79.26
5 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Kodaq DNA Polymerase is the solution for a robust PCR system with high fidelity and yield.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Catalog No. ABIN4219111
$79.26
250 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Proteinase K is commonly used in molecular biology to remove protein contamination.

Application:
DNA Isolation, Endotoxin Removal, Immunohistochemistry
Concentration:
20 mg/mL
Storage:
4 °C
Unit Definition:
One unit is defined as the enzyme activity that produce1 µmol of tyrosine per minute from casein at 37°C at pH 7.5. Refer to the certificate of analysis for specific values for the present lot.
Specificity:
DNase is not detected in quality control procedure of incubation 40 µg Proteinase K with 1 µg λ DNA for 6 hours at 37°C.
RNase is not detected in quality control procedure of incubation 40 µg Proteinase K with 2 µg RNA for 2 hours at 37°C. This preparation is considered as RNase and DNase free.
Each lot was tested to ensure the absence of Nucleases and DNA.
Catalog No. ABIN6383959
$80.00
2 mL
Plus shipping costs $45.00
Delivery in 11 to 13 Business Days
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TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 6X DNA Loading Buffer, 50 mM MgSO2
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519545
$80.00
100 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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The Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.

Storage:
-20 °C,-80 °C
Unit Definition:
One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Catalog No. ABIN5666462
$80.00
1000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days