Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

351 Products
Data Quality
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Type
  • 351
    Enzyme
Gene
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  • 2
  • 2
  • 1
Species
  • 8
  • 5
  • 4
  • 2
  • 1
Application
  • 123
  • 66
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Host
  • 11
  • 4
Protein Type
  • 9
Format
  • 22
  • 4

Application:
Polymerase Chain Reaction
Components:
2xTransTaq®-T PCR SuperMix (+dye), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Catalog No. ABIN5519418
$89.36
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
Polymerase Chain Reaction
Components:
2xTransTaq®-T PCR SuperMix (-dye), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Catalog No. ABIN5519420
$89.36
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
Polymerase Chain Reaction
Components:
Bst DNA Polymerase, Large Fragment 8.0 unit/μl, 10x BstPol Reaction Buffer, 100 mM MgSO4
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
Catalog No. ABIN5666435
$90.00
2000 U
Plus shipping costs $45.00
Will be delivered in 31 Business Days

Application:
Polymerase Chain Reaction
Components:
2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (-dye ), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb.
Catalog No. ABIN5519414
$90.82
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
Polymerase Chain Reaction
Components:
2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (+dye ), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb.
Catalog No. ABIN5519416
$90.82
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^CATGG...3'
3'...GGATC^C...5'
Catalog No. ABIN5519444
$90.82
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Catalog No. ABIN5519574
$90.82
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
DNA Modification
Concentration:
10 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Nt.BstNBI (Bst Nickase) that digests 1 μg of T7 DNA into 5 distinct bands (13, 9, 7, 6, and 4 kb) in 1 hour at 55°C in a reaction volume of 50 μl.
Catalog No. ABIN3188203
$92.21
1000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188219
$92.21
50 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188221
$92.21
50 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188223
$92.21
50 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188225
$92.21
50 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188227
$92.21
50 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188184
$92.21
50 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188186
$92.21
50 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
1000 nM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188188
$92.21
50 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Publications
  • Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
  • Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
Catalog No. ABIN4219169
$92.21
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
M-MLV Reverse Transcriptase (RNaseH-)200U/μl, 5x M-MLV Reaction Buffer
Catalog No. ABIN5666450
$94.00
5000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
ISO 9001:2008

Application:
DNA Modification
Concentration:
250 U/μL
Storage:
-20 °C
Specificity:
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 ℃, which corresponds approximately to complete digestion of 37 μg DNA. Note that 1 KU=1000 units.
Catalog No. ABIN2180646
$96.80
Plus shipping costs $45.00
Will be delivered in 5 Business Days

Application:
DNA Manipulation
Concentration:
8 U/μL
Components:
Enzyme supplied with 2X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Bst DNA Polymerase, Large Fragment that that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
Catalog No. ABIN3188182
$98.68
1600 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days