Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

Data Quality
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Type
  • 220
    Enzyme
Gene
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Species
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  • 1
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Application
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Host
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Protein Type
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Format
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220 Products

Application:
cDNA Synthesis
Components:
EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519431
$101.58
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...CGAT^CG...3'
3'...GC^TAGC...5'
Catalog No. ABIN5519470
$101.58
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519554
$101.58
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519557
$104.44
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Ligation
Concentration:
1 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188209
$105.15
200 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Ligation
Concentration:
10 U/μL
Catalog No. ABIN3188212
$105.15
100 μL
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
A combination of chemical blocking technique with TransStart® hot start technique to achieve complete blocking. Compared with double blocking TransStart® TopTaq, this method provides higher sensitivity, higher specificity, better amplification.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519560
$107.31
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® TopTaq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519564
$107.31
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Restriction Digest
Concentration:
20 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Dpn1 required to digest 1 μg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Catalog No. ABIN3188191
$111.62
2000 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
RNA Modification
Concentration:
50 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume in 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer.
Catalog No. ABIN3188215
$111.62
100 μL
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519568
$113.04
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519571
$115.90
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
UDG and dUTP avoid cross contamination.
Catalog No. ABIN5519542
$121.63
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Polymerase Chain Reaction
Components:
2xTransStart® FastPfu PCR SuperMix (-dye ), ddH2O
Storage:
-20 °C
Specificity:
TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 2-4 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 15 kb.
Amplification of plasmid DNA fragment up to 20 kb.
Catalog No. ABIN5519412
$121.63
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Catalog No. ABIN5519440
$122.89
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 6X DNA Loading Buffer, 50 mM MgSO2
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519545
$124.50
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
10 μM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188185
$124.56
40 μg
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
10 μM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188187
$124.56
40 μg
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Ligation
Concentration:
10 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of E. coli DNA Ligase that is required to give 50% ligation of HindIII-digested λ DNA in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E.coli DNA Ligase Reaction Buffer (concentration of 5' DNA termini is 0.12 μM [300 μg/ml]).
Catalog No. ABIN3188192
$124.56
1000 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
SalI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188208
$124.56
10000 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days