Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

351 Products
Data Quality
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Type
  • 351
    Enzyme
Gene
  • 5
  • 3
  • 2
  • 2
  • 1
Species
  • 8
  • 5
  • 4
  • 2
  • 1
Application
  • 123
  • 66
  • 38
  • 33
  • 24
Host
  • 11
  • 4
Protein Type
  • 9
Format
  • 22
  • 4
Supplier: Log in to see

Application:
Polymerase Chain Reaction
Components:
Taq DNA Polymerase 5 U/μl, 10xTaq PCR Buffer, 25 mM MgCl2
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666456
$100.00
500 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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Application:
cDNA Synthesis
Components:
EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519431
$100.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519551
$101.02
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519539
$101.02
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519554
$102.48
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...CGAT^CG...3'
3'...GC^TAGC...5'
Catalog No. ABIN5519470
$102.48
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Storage:
-20 °C,-80 °C
Unit Definition:
One unit of the enzyme is the amount that completely cleaves 1 μg ds-DNA with dU in one hour at 37°C in the condition of 20 mM Tris-Hcl (PH8.0), 1 mM EDTA, 10 mM NaCl.
Catalog No. ABIN5666462
$105.00
1000 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
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Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
4 °C/-20 °C
Specificity:
The use of Taq Plus DNA Polymerase in this MasterMix offers:
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
Catalog No. ABIN4219147
$105.15
5 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Ligation
Concentration:
1 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188209
$105.15
200 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains EasyScript™ Plus Reverse Transcriptase.
Publications
  • Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
Catalog No. ABIN4219171
$105.15
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Polymerase Chain Reaction
Storage:
-20 °C
Specificity:
With Safe-Green™ 2X PCR Taq MasterMix, you can
- Reduce PCR set up time
- Eliminate ethidium bromide usage and associated waste disposal costs
- Increase PCR efficiency and reproducibility

The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219178
$105.15
5 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Ligation
Concentration:
10 U/μL
Catalog No. ABIN3188212
$105.15
100 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
4 °C/-20 °C
Specificity:
The use of Taq Plus DNA Polymerase in this MasterMix offers:
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
Catalog No. ABIN4219146
$105.15
5 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
Supplier: Log in to see

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519557
$105.39
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
Supplier: Log in to see

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
TransStart® TopTaq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519564
$108.31
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
A combination of chemical blocking technique with TransStart® hot start technique to achieve complete blocking. Compared with double blocking TransStart® TopTaq, this method provides higher sensitivity, higher specificity, better amplification.
Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519560
$108.31
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
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Application:
RNA Modification
Concentration:
50 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume in 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer.
Catalog No. ABIN3188215
$111.62
5000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Dpn1 required to digest 1 μg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Catalog No. ABIN3188191
$111.62
2000 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Polymerase Chain Reaction, Mutagenesis
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Precision™ DNA Polymerase, you can expect
- Outstanding accuracy and efficiency
- Error-free PCR products for downstream processes
Catalog No. ABIN4219102
$111.62
500 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
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Application:
Quantitative real-time PCR
Catalog No. ABIN3188317
$113.00
200 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 6 to 7 Business Days