Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

Data Quality
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Type
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    Enzyme
Gene
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Species
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  • 1
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Application
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Host
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Protein Type
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Format
  • 16
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220 Products

Application:
Polymerase Chain Reaction
Components:
2xTransStart FastPfu Fly PCR SuperMix, ddH2O
Storage:
-20 °C
Specificity:
TransStart® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 2-6 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 15 kb.
Amplification of plasmid DNA fragment up to 20 kb.
Catalog No. ABIN5519410
$126.32
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519548
$127.36
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Pfu Buffer, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Catalog No. ABIN5519527
$128.80
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Catalog No. ABIN5519530
$131.66
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519523
$135.96
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
RNA Modification
Concentration:
1 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.
Catalog No. ABIN3188207
$137.50
100 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
EasyScript® RT/RI Enzyme Mix, 2xES Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the, product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519428
$141.69
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
Ligation
Concentration:
5 U/μL
Storage:
-80 °C
Specificity:
An enzyme which anneals DNA strands together
Catalog No. ABIN411759
$150.00
250 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Pfu Buffer, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Catalog No. ABIN5519533
$150.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 12 to 16 Business Days

Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188210
$150.44
500 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Catalog No. ABIN5519442
$152.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519502
$153.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519521
$153.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Catalog No. ABIN5519536
$153.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
NotI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188202
$156.91
10000 U
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519500
$178.00
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 15 to 19 Business Days

Application:
RNA Modification
Catalog No. ABIN2953155
$184.62
25 mg
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days

Application:
Polymerase Chain Reaction
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952618
$184.62
Plus shipping costs $45.00
Delivery in 11 to 16 Business Days

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219166
$182.79
100 x 20 μL
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days

Application:
Genome Editing with Engineered Nucleases
Concentration:
10 μM
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188189
$189.26
40 μg
Plus shipping costs $45.00 and $24.00 dry ice
Delivery in 3 to 4 Business Days