Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

286 Products
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    Enzyme
Gene
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Species
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Host
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Application
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^CCGGG...3'
3'...GGGCC^C...5'
Catalog No. ABIN5519488
$34.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^GGCGG...3'
3'...GGCGG^C...5'
Catalog No. ABIN5519450
$35.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...G^CTAGC...3'
3'...CGATC^G...5'
Catalog No. ABIN5519464
$35.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...C^CATGG...3'
3'...GGATC^C...5'
Catalog No. ABIN5519444
$43.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Extraction-free DNA amplification from whole blood samples with Bloodirect DNA Polymerase.

Application:
Polymerase Chain Reaction
Concentration:
1 U/μL
Storage:
-20 °C
Specificity:
With Bloodirect DNA Polymerase, you can :
- Eliminate lengthy DNA purification steps
- Achieve direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin
Catalog No. ABIN4219173
$43.03
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Catalog No. ABIN5519577
$46.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219164
$53.38
25 reactions
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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Super fast restriction enzyme

Application:
Restriction Digest
Components:
Enzyme, 10X Buffer, 10X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Specificity:
5'...CGAT^CG...3'
3'...GC^TAGC...5'
Catalog No. ABIN5519470
$55.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.

Application:
cDNA Synthesis
Components:
EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519431
$55.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519557
$58.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Short on time? TaqFast DNA Polymerase to the rescue.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With TaqFast DNA Polymerase, you can expect:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80 %
Catalog No. ABIN4219104
$59.85
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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Struggling to amplify long targets? Long-Range DNA Polymerase will end your fight.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Long-Range DNA Polymerase, you can expect:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Efficient amplification of DNA templates up to 20kb
Catalog No. ABIN4219108
$66.32
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519571
$71.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219163
$72.79
25 reactions
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Catalog No. ABIN5519440
$73.15
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Bestaq™ DNA Polymerase is the new standard.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Bestaq™ DNA Polymerase, you can expect
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Catalog No. ABIN4219106
$79.26
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days
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TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519548
$83.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
Supplier: Log in to see

EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Catalog No. ABIN5519442
$102.60
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Catalog No. ABIN5519536
$111.00
Plus shipping costs $45.00 and $23.00 dry ice
Will be delivered in 15 to 19 Business Days
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Frustrated with amplification errors? Let us introduce you to Precision™ DNA Polymerase.

Application:
Polymerase Chain Reaction, Mutagenesis
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Precision™ DNA Polymerase, you can expect
- Outstanding accuracy and efficiency
- Error-free PCR products for downstream processes
Catalog No. ABIN4219102
$111.62
500 U
Plus shipping costs $45.00
Will be delivered in 3 to 4 Business Days