Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

258 Products
Data Quality
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Type
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    Enzyme
Gene
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Species
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Host
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Application
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Protein Type
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Components:
25 x 1.0 ml vials
Storage:
-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219184
$229.17
25 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability.

Application:
cDNA Synthesis
Components:
TransScript® II Reverse Transcriptase, 5xTS II RT Buffer, Anchored Oligo (dT)20 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Increased thermostability for more full-length cDNA products.
Reaction temperature at 42 °C-55 °C.
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb.
Catalog No. ABIN5519516
$231.00
10000 units
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Hot Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Hot Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer, pH 8.0 (19 °C).
Storage:
-20 °C
Specificity:
Hot Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188333
$235.20
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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HGS Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
HGS Diamond Taq® DNA Polymerase is provided at a concentration > 5 U/μl with Reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) and MgCl2 solution (25 mM MgCl2).
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, after activation step, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
HGS Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188330
$313.60
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Components:
25 x 1.0 ml vials
Storage:
-20 °C
Specificity:
The use of Taq Plus DNA Polymerase in this MasterMix offers:
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
Catalog No. ABIN4219187
$351.39
25 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Components:
25 x 1.0 ml vials
Storage:
-20 °C
Specificity:
The use of Taq Plus DNA Polymerase in this MasterMix offers:
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
Catalog No. ABIN4219186
$351.39
25 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Routine amplification in a cost-effective way? Taq DNA Polymerase is the answer.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Taq DNA Polymerase, you can expect
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219103
$363.61
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Application:
DNA Modification
Concentration:
>1800 U/mg
Catalog No. ABIN1543785
$385.00
50 kU
Plus shipping costs $45.00 Will be delivered in 2 to 3 Business Days
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UNG efficiently hydrolyzes the release of free uracil from uracil-containing single stranded or double stranded DNA, but not from oligomers (6 or less bases).

Application:
Quantitative real-time PCR
Concentration:
5000 units/mL
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double stranded, uracil containing DNA.
Catalog No. ABIN3188315
$740.25
1500 U
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 6 to 7 Business Days
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High Efficiency Taq Mastermix

Application:
Polymerase Chain Reaction
Components:
2xEasyTaq® PCR SuperMix ( +dye ), ddH2O
Storage:
-20 °C
Specificity:
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 4 kb.
Catalog No. ABIN5519400
$26.60
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Special Buffer for PAGE

Application:
Polymerase Chain Reaction
Components:
2xEasyTaq® PCR SuperMix for PAGE (+dye ), ddH2O
Storage:
-20 °C
Specificity:
Extension rate is about 1-2 kb/min.
Unique buffer system compatible with PAGE.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 3 kb.
Catalog No. ABIN5519407
$26.60
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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suitable for high fidelity TA cloning

Application:
Polymerase Chain Reaction
Components:
2xTransTaq®-T PCR SuperMix (+dye), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Catalog No. ABIN5519418
$42.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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ready-to-use Pfu PCR SuperMix

Application:
Polymerase Chain Reaction
Components:
2xEasyPfu PCR SuperMix (-dye ), ddH2O
Storage:
-20 °C
Specificity:
EasyPfu PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 0.5 kb/min.
PCR products can be directly cloned into pEASY®-Blunt vectors.
Amplification of genomic DNA fragment up to 6 kb.
Amplification of plasmid DNA fragment up to 10 kb.
Catalog No. ABIN5519398
$42.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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suitable for high fidelity TA cloning

Application:
Polymerase Chain Reaction
Components:
2xTransTaq®-T PCR SuperMix (-dye), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb.
Catalog No. ABIN5519420
$42.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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ready-to-use mixture for the amplification of genomic DNA

Application:
Polymerase Chain Reaction
Components:
2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (-dye ), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb.
Catalog No. ABIN5519414
$43.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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ready-to-use mixture for the amplification of genomic DNA

Application:
Polymerase Chain Reaction
Components:
2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (+dye ), ddH2O
Storage:
-20 °C
Specificity:
TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb.
Catalog No. ABIN5519416
$43.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
4 °C/-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219145
$76.39
5 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
4 °C/-20 °C
Specificity:
The use of Taq DNA Polymerase in this MasterMix offers:
- Robust PCR performance with great reproducibility
- High sensitivity
Catalog No. ABIN4219144
$76.39
5 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Catalog No. ABIN5519530
$88.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.

Application:
cDNA Synthesis
Components:
TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Storage:
-20 °C
Unit Definition:
One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519523
$92.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days