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TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

cDNA
Catalog No. ABIN5519498
  • Application
    cDNA Synthesis (cDNA)
    Purpose
    The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA.
    Brand
    TransScript®
    Specificity
    Simultaneous genomic DNA removal and cDNA synthesis.
    The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
    qPCR ready cDNA in 15 minutes.
    cDNA up to 250 bp.
    Characteristics
    It is provided at 5x concentration and used at 1x concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85oC for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.
    Components
    5xTransScript® All-in-One SuperMix for PCR, 5xTransScript® All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
  • Application Notes
    Multiple copy and low copy gene detection
    Comment

    50 rxnsx20 μl Systems

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    at -20°C for two years
    Expiry Date
    24 months
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