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TransStart® FastPfu DNA Polymerase (with 2.5 mM dNTPs)

PCR
Catalog No. ABIN5519530
  • Application
    Polymerase Chain Reaction (PCR)
    Purpose
    TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
    Brand
    TransStart®
    Characteristics
    - Extension rate is about 2-4 kb/min.
    - TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
    - PCR products can be directly cloned into pEASY®-Blunt vectors.
    - Amplification of genomic DNA fragment up to 15 kb.
    - Amplification of plasmid DNA fragment up to 20 kb.
    Components
    DNA Polymerase, 10X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
    Unit Definition
    One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
  • Application Notes
    High fidelity PCR, High yield and fast PCR, Blunt end cloning, Site-directed mutagenesis, Complex templates
    Comment

    TransStart® FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

    Restrictions
    For Research Use only
  • Buffer
    Storage Buffer: 50 mM Tris-HCl ( pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50 % glycerol
    5xTransStart® FastPfu Buffer with 20 mM MgSO4: 100 mM Tris-SO4 ( pH 9.2), 50 mM (NH4)2SO4, 200 mM KCl, 10 mM MgSO4, 10 % Glycerol, others
    Storage
    -20 °C
    Storage Comment
    at -20°C for two years
    Expiry Date
    24 months
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