TransScript® II One-Step gDNA Removal and cDNA Synthesis SuperMix
cDNA
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- Application
- cDNA Synthesis (cDNA)
- Purpose
- Unique genomic DNA remover is combined with TransScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.
- Brand
- TransScript®
- Specificity
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Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 15 kb. - Characteristics
- After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85 °C for 5 seconds.
- Components
- TransScript® II RT/RI Enzyme Mix, gDNA Remover, 2xTS II Reaction Mix, Random Primer (0.1 μg/μL), Anchored Oligo(dT)20 Primer (0.5 μg/μL), RNase-free Water
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- Application Notes
- cDNA library construction, 3' and 5' RACE, Multiple copy and low copy gene detection, GC-rich or complex secondary structure RNA template
- Comment
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50 rxnsx20 μl Systems
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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