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TransScript® Reverse Transcriptase

cDNA
Catalog No. ABIN5519523
  • Application
    cDNA Synthesis (cDNA)
    Purpose
    TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.
    Brand
    TransScript®
    Specificity
    Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
    Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
    cDNA up to 12 kb.
    Components
    TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
    Unit Definition
    One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
  • Application Notes
    First-strand cDNA synthesis, Multiple copy and low copy gene detection
    Restrictions
    For Research Use only
  • Buffer
    5xTS RT Buffer: 250 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4
    Storage
    -20 °C
    Storage Comment
    at -20°C for two years
    Expiry Date
    24 months
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