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TransScript® II Reverse Transcriptase

cDNA
Catalog No. ABIN5519516
$345.00
Plus shipping costs $45.00 and $20.00 dry ice
10000 units
Shipping to: United States
Delivery in 12 to 16 Business Days
  • Application
    cDNA Synthesis (cDNA)
    Purpose
    TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability.
    Brand
    TransScript®
    Specificity
    Increased thermostability for more full-length cDNA products.
    Reaction temperature at 42 °C-55 °C.
    Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
    Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
    cDNA up to 15 kb.
    Characteristics
    The enzyme is active at up to 55 °C. It provides higher specificity, higher yield and more full-length cDNA products.
    Components
    TransScript® II Reverse Transcriptase, 5xTS II RT Buffer, Anchored Oligo (dT)20 Primer (0.5 μg/μL)
    Unit Definition
    One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
  • Application Notes
    First-strand cDNA synthesis, cDNA library construction, 3' and 5' RACE, Multiple copy and low copy gene detection, GC-rich or complex secondary structure RNA template
    Restrictions
    For Research Use only
  • Buffer
    10xTS II RT Buffer: 500 mM KCl, 30 mM MgCl2, 100 mM Tris-HCl pH 8.4
    Storage
    -20 °C
    Storage Comment
    at -20°C for two years
    Expiry Date
    24 months
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