TransScript® II Reverse Transcriptase
cDNA
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- Application
- cDNA Synthesis (cDNA)
- Purpose
- TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability.
- Brand
- TransScript®
- Specificity
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Increased thermostability for more full-length cDNA products.
Reaction temperature at 42 °C-55 °C.
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb. - Characteristics
- The enzyme is active at up to 55 °C. It provides higher specificity, higher yield and more full-length cDNA products.
- Components
- TransScript® II Reverse Transcriptase, 5xTS II RT Buffer, Anchored Oligo (dT)20 Primer (0.5 μg/μL)
- Unit Definition
- One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
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- Application Notes
- First-strand cDNA synthesis, cDNA library construction, 3' and 5' RACE, Multiple copy and low copy gene detection, GC-rich or complex secondary structure RNA template
- Restrictions
- For Research Use only
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- Buffer
- 10xTS II RT Buffer: 500 mM KCl, 30 mM MgCl2, 100 mM Tris-HCl pH 8.4
- Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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