Phi29 DNA Polymerase

Details for Product No. ABIN3188235,
DNA Amplification (DNA Amp)
Characteristics Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3'→5' exonuclease activity and can amplify from very small amounts of starting templates.
Components Enzyme supplied with 5X Reaction Buffer
Unit Definition One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.

  • Rolling circle amplification (RCA)
  • Multiple displacement amplification (MDA)
  • Whole genome amplification (WGA)
  • DNA template preparation for sequencing
  • Proteinprimed DNA amplification

Restrictions For Research Use only
Concentration 10 U/μL
Buffer 50 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5 % Tween-20, 0.5 % NP-40 and 50 % (v/v) Glycerol.
Storage -20 °C