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Phi29 DNA Polymerase

DNA Amp 10 U/μL
Catalog No. ABIN3188235
  • Application
    DNA Amplification (DNA Amp)
    Characteristics
    Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3'→5' exonuclease activity and can amplify from very small amounts of starting templates.
    Components
    Enzyme supplied with 5X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
  • Comment

    • Rolling circle amplification (RCA)
    • Multiple displacement amplification (MDA)
    • Whole genome amplification (WGA)
    • DNA template preparation for sequencing
    • Proteinprimed DNA amplification

    Restrictions
    For Research Use only
  • Concentration
    10 U/μL
    Buffer
    50 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5 % Tween-20, 0.5 % NP-40 and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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