EasyScript® Reverse Transcriptase
cDNA
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- Application
- cDNA Synthesis (cDNA)
- Purpose
- EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.
- Brand
- EasyScript®
- Specificity
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Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb. - Characteristics
- The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.
- Components
- EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
- Unit Definition
- One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
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- Application Notes
- First-strand cDNA synthesis, Multiple copy gene detection
- Restrictions
- For Research Use only
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- Buffer
- 5xES RT Buffer: 375 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4
- Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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