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PHOENIXDX® MYCOPLASMA MIX...
Optional: Perform a UNG-digestion according to the manufacturer's guidelinesProgram your PCR instrument.Set FAM as reporter for mycoplasma detection, HEX for detection of the PCR Positive Control (PPC) and (if required) ROX for passive reference.Initial Denaturation: (1 Cycle) 95 °C 5 minAmplification: (50 Cycles) 95 °C 15sec, 52 °C 1 minSetup your PCR reactions in suitable PCR disposables:PhoenixDx® Mycoplasma Mix: Volume 10 μL Final Conc. 1XTemplate DNA/culture supernatant: Volume X μl Final Conc. 1-50 ngNuclease-free dH2O: Volume X μl Final Conc. to 20 μLLoad the reactions into your PCR device and start the program.
Storage: -20 °C
storageComment: Store all components at -20°C and avoid repeated freeze and thaw cycles.