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TransTaq®-T DNA Polymerase Enzyme

PCR
Catalog No. ABIN5519580
$77.22
Plus shipping costs $45.00 and $20.00 dry ice
local_shipping Shipping to: United States
Delivery in 12 to 16 Business Days
  • Application
    Polymerase Chain Reaction (PCR)
    Purpose
    TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease.
    Brand
    TransTaq®
    Specificity
    TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.
    Characteristics
    - TransTaq®-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
    - Extension rate is about 1-2 kb/min.
    - Template-independent"A"can be generated at the 3'end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
    - Amplification of genomic DNA fragment up to 8 kb.
    Components
    DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
    Unit Definition
    One unit of TransTaq®-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
  • Application Notes
    Complex templates, TA Cloning
    Comment

    TransTaq®-T DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq®-T DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

    Restrictions
    For Research Use only
  • Buffer
    Storage Buffer: 20 mM Tris-HCl ( pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50 % glycerol, stabilizers
    10xTransTaq®-T Buffer: 200 mM Tris-HCl ( pH 9.0), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others
    Storage
    -20 °C
    Storage Comment
    at -20°C for two years
    Expiry Date
    24 months
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