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T4 DNA Ligase Enzyme

Lig 1 U/μL
Catalog No. ABIN3188209
$105.15
Plus shipping costs $45.00 and $20.00 dry ice
200 U
local_shipping Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    Ligation (Lig)
    Characteristics
    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3'- hydroxyl and 5'- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
    Components
    Enzyme supplied with 5X Reaction Buffer
  • Comment

    • Cloning of restriction fragments
    • Attaching doublestranded oligonucleotide linkers or adaptors to DNA
    • Sitedirected mutagenesis
    • Nick repair of duplex DNA, RNA or DNARNA hybrids
    • Selfcircularization of linear DNA

    Restrictions
    For Research Use only
  • Concentration
    1 U/μL
    Storage
    -20 °C
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