Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

343 Products
Data Quality
  • 18
  • 21
Type
  • 343
    Enzyme
Gene
  • 4
  • 2
  • 2
  • 2
  • 1
Species
  • 8
  • 4
  • 3
  • 2
  • 1
Application
  • 123
  • 66
  • 33
  • 33
  • 24
Host
  • 11
  • 4
Protein Type
  • 9
Format
  • 19
  • 4

Catalog No. ABIN5684240
$287.00
100 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days

Components:
EvoTM Reverse Transcriptase (200 U/μl), 5X RT buffer
Storage:
-20 °C
Catalog No. ABIN5684178
$287.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days

Application:
Polymerase Chain Reaction
Components:
FlashPfu DNA Polymerase 1 U/μl, 2x flashPfu PCR Buffer, 25 mM MgSO4
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666440
$294.00
2 x 250 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days

Application:
Quantitative real-time PCR
Storage:
-20 °C
Specificity:
gDNA remover and 5xTransScript® All-in-One SuperMix for qPCR are provided for simultaneous gDNA removal and cDNA synthesis.
TransStart® Tip Green qPCR SuperMix is provided for qPCR.
5xTransScript® All-in-One No-RT Control SuperMix for qPCR is provided for experimental control.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519504
$298.00
1 kit
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® One-Step Enzyme Mix, 2x One-Step Reaction Mix, RNase-free Water
Storage:
-20 °C
Specificity:
Amplification of fragment up to 8 kb.
Catalog No. ABIN5519518
$302.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
Restriction Digest
Components:
50 Units PvuRts1 I restriction enzyme
10X PvuRts1 I Reaction Buffer
1 M DTT
Storage:
-20 °C/-80 °C
Specificity:
PvuRts1 I restriction enzyme recognizes the sequence hmCN11-12/N9-10G.
Catalog No. ABIN2669403
$303.33
50 units
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 1 to 2 Business Days

Application:
cDNA Synthesis
Components:
5x TransScript® All-in-One SuperMix for PCR, RNase-free Water
Storage:
-20 °C
Specificity:
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb.
Catalog No. ABIN5519508
$305.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
RNA Modification
Concentration:
20 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of RNase R that converts 1 μg of poly(A) into acid-soluble nucleotides in 10 minutes at 37°C.
Catalog No. ABIN3188237
$312.21
500 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days

Components:
NovoTM Reverse Transcriptase (200 U/μl), 5X RT Buffer
Storage:
-20 °C
Catalog No. ABIN5684224
$313.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684173
$313.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684186
$313.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684172
$313.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days

Application:
Polymerase Chain Reaction
Storage:
4 °C,-20 °C
Catalog No. ABIN5684185
$313.00
200 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 2 to 3 Business Days

Application:
Ligation
Catalog No. ABIN1543742
$316.92
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days

Application:
Protein Extraction
Concentration:
250 U/μL
Storage:
4 °C
Unit Definition:
According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37°C.
Catalog No. ABIN6383966
$341.00
Plus shipping costs $45.00
Delivery in 11 to 13 Business Days

Application:
cDNA Synthesis
Components:
C to C Lysis Buffer, Enzyme Mix, gDNA Remover, Reaction Mix, Oligo (dT)/RP Mix, RNase-free Water
Storage:
-20 °C
Catalog No. ABIN5519494
$342.00
25 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
cDNA Synthesis
Components:
TransScript® II RT/RI Enzyme Mix, gDNA Remover, 2xTS II Reaction Mix, Random Primer (0.1 μg/μL), Anchored Oligo(dT)20 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 15 kb.
Catalog No. ABIN5519514
$342.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
Ligation
Components:
BirA, 10xBirA Buffer A, 10xBirA Buffer B, D-Biotin, manual
Storage:
-80 °C
Catalog No. ABIN5666437
$355.00
50 μg
Plus shipping costs $45.00 and $22.60 dry ice
Will be delivered in 31 Business Days

Application:
cDNA Synthesis
Components:
5xTransScript® II All-in-One SuperMix for PCR, 5xTransScript® II All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of Oligo(dT)20 Primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Catalog No. ABIN5519507
$356.00
50 reactions
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
HGS Diamond Taq® DNA Polymerase is provided at a concentration > 5 U/μl with Reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) and MgCl2 solution (25 mM MgCl2).
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, after activation step, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
HGS Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188330
$365.75
Plus shipping costs $45.00
Delivery in 6 to 7 Business Days