BenzNuclease DNA and RNA Nuclease (Ultra Pure,Protease Free)

Reaction buffer:50 mM Tris-HCl, 1 mM MgCl2, pH 8.0 （In the case of extensive dilution before use, carrier protein such as 0.1 mg/ml HSA or BSA is generally recommended to avoid any enzyme loss from surface adsorption)
DNA Substrate: 1 mg/ml salmon sperm DNA is dissolved overnight at 4 ℃, in reaction buffer, and is then sonicated on ice to obtain a homogenous solution.
Enzyme: Different dilution of nuclease with reaction buffer
Stop reagent: Trichloroacetic acid (TCA)

Measurement of activity: The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of BenzNuclease is >1.5 x 10 e6 unit/mg protein.

Standard curve establishment:
- 400 μl substrate + 100 μl enzyme of known activity = 500 μl mixture
- Incubate the mixture at 37℃ for 30 min.
- Stop the reaction by addition of 400 μl cold TCA and incubate on ice for 10 min.
- Centrifuge at 8500 g for 5 min.
- Measure the absorbance of supernatant at 260 nm.
- Lot a standard curve with nuclease of known activities for each set of measurements.