HEK 293 Host Cell DNA Residue Detection Kit
qPCR
Species: Human
Quantitative
Quick Overview for HEK 293 Host Cell DNA Residue Detection Kit (ABIN7538043)
Gene
Host Cell DNA
Species
Human
Application
Quantitative real-time PCR (qPCR)
Analytical Method
Quantitative
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Detection Range
- 30 fg/μL - 3000 pg/μL
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Minimum Detection Limit
- 30 fg/μL
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Purpose
- The HEK293 Host Cell DNA Residue Detection Kit can be used for Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell.
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Characteristics
- This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 30fg/μL level. The preparation process of DNA Control is completely consistent with that of other host cell DNA national standards, therefore it has high purity and no protein and ion interference to ensure the accuracy of the sample quantitative detection. The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.
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Components
- 2XqPCR Mix(UDG PLUS), Primer&Probe Mix, DNA Dilution Buffer, DNA Control (30ng/μL), RNase-Free H2O, 50X ROX Reference Dye (Optional)
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Protocol
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- 2XqPCR Mix: 15.125 μL
- Primer&Probe Mix: 2 μL
- DNA template (control or sample): 5 μL
- Add water: 2.875 μL
- Total Volume: 25 μL
The detection range of the standard curve mentioned above is suitable for most experiments and can be adjusted as needed, such as 500pg/μL-50fg/μL. -
Assay Precision
- Intra Variation% 10.3-12.3, Inter Variation% 16-19
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Restrictions
- For Research Use only
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Storage
- -20 °C
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- Host Cell DNA
Target
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