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Mouse DCTN6 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)

This is a Dynactin 6 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: 330 bp. Transient expression. Suitable for PExp. Bacterial selection: Kanamycin. Grade: End-sequenced.
OriGene
Catalog No. ABIN3298404
Supplier Product No.: mc225679
$163.35
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 16 to 17 Business Days

Quick Overview for Mouse DCTN6 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3298404)

Gene

DCTN6 (Dynactin 6 (DCTN6))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-Entry

Promoter

Enhanced CMV Promoter

Bacterial Resistance

Kanamycin

Expression Type

Transient
  • Species

    Mouse

    Supplier Product No.

    mc225679

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Mouse (Murine) Dctn6 is ideal for over-expression of native protein for functional studies.

    Specificity

    Restriction Site: SgfI-MluI. With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Grade

    End-sequenced

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Insert Length

    330 bp

    Selectable Marker

    Neomycin

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Target

    DCTN6 (Dynactin 6 (DCTN6))

    Alternative Name

    Dctn6

    NCBI Accession

    NM_001293759, NP_001280688
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