Mouse AKAP1 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a A Kinase (PRKA) Anchor Protein 1 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: 2574 bp. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin. Grade: End-sequenced.
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Catalog No. ABIN3309312
Supplier Product No.: mc222137
$855.36
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Mouse AKAP1 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3309312)
Gene
AKAP1
(A Kinase (PRKA) Anchor Protein 1 (AKAP1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Mouse
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Supplier Product No.
- mc222137
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Mouse (Murine) Akap1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: SgfI-MluI. With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Grade
- End-sequenced
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2574 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))
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Alternative Name
- Akap1
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Background
- Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif. [UniProtKB/Swiss-Prot Function]
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NCBI Accession
- NM_009648, NP_033778
Target
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