Human ACTB cDNA Clone in Mammalian Expression Vector
This is a Actin, beta plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: 1800 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3317194
Supplier Product No.: sc319328
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ACTB cDNA Clone in Mammalian Expression Vector (ABIN3317194)
Gene
beta Actin (ACTB)
(Actin, beta (ACTB))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc319328
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ACTB is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: EcoRI-XhoI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1800 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Inducing iron deficiency improves erythropoiesis and photosensitivity in congenital erythropoietic porphyria." in: Blood, Vol. 126, Issue 2, pp. 257-61, (2015) (PubMed).
: "Rapamycin regulates stearoyl CoA desaturase 1 expression in breast cancer." in: Molecular cancer therapeutics, Vol. 9, Issue 10, pp. 2770-84, (2010) (PubMed).
: "Altered podocyte structure in GLEPP1 (Ptpro)-deficient mice associated with hypertension and low glomerular filtration rate." in: The Journal of clinical investigation, Vol. 106, Issue 10, pp. 1281-90, (2000) (PubMed).
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: "Inducing iron deficiency improves erythropoiesis and photosensitivity in congenital erythropoietic porphyria." in: Blood, Vol. 126, Issue 2, pp. 257-61, (2015) (PubMed).
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- beta Actin (ACTB) (Actin, beta (ACTB))
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Alternative Name
- ACTB
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Background
- This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_001101, NP_001092
Target
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