Human DES cDNA Clone in Mammalian Expression Vector
This is a Desmin plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: 2300 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3317480
Supplier Product No.: sc319574
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human DES cDNA Clone in Mammalian Expression Vector (ABIN3317480)
Gene
Desmin (DES)
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc319574
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human DES is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: EcoRI-XhoI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2300 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Targeted analysis of whole genome sequence data to diagnose genetic cardiomyopathy." in: Circulation. Cardiovascular genetics, Vol. 7, Issue 6, pp. 751-9, (2014) (PubMed).
: "De novo desmin-mutation N116S is associated with arrhythmogenic right ventricular cardiomyopathy." in: Human molecular genetics, Vol. 19, Issue 23, pp. 4595-607, (2010) (PubMed).
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- Desmin (DES)
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Alternative Name
- DES
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Background
- This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_001927, NP_001918
Target
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